Search

Objectives: To establish the existence of concurrent infections by different dengue virus (DENV) serotypes in an outbreak in the Northwestern in Peru during 2008., Material and Methods: 73 serum samples from patients with dengue were analyzed during an outbreak that occurred in Northwestern in Peru between May and June 2008. Molecular biology techniques were used to serotype the DENV, thus, firstly the viral RNA viral was extracted using Viral QIAamp RNA mini kit (Qiagen, Valencia, California, USA), then the viral cDNA fragments were reverse transcripted and amplified by means of the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and the RT-Nested PCR region techniques and finally, genetic sequencing of the viral cDNA fragments were performed using the Big Dye Terminator v.3,1 kit., Results: The 73 dengue cases presented infections by different serotypes: 34 (46.6%) by DENV-3, 29 (39.7%) by DENV-1, 4 (5.5%) by DENV-4, and 6 (8.2%) concurrent infections by DENV-1 and DENV-3. The most frequent clinical manifestations observed among dengue patients were fever and headache (100%), myalgia (94.5%), ocular pain (83.6%), arthralgia (78.1%), shivers (63.0%), nausea/vomiting (38.4%), positive tourniquet test (30.1%), and rash (20.5%). All patients with concurrent infections presented light clinical course of dengue fever (DF) except one patient who had moderate hemorrhagic manifestations., Conclusion: This is the first Peruvian report of patients with concurrent infections of two DENV serotypes without severe clinical manifestations.

Objetives. To establish the existence of concurrent infections by different dengue virus (DENV) serotypes in an outbreak in the Northwestern in Peru during 2008. Material and methods. 73 serum samples from patients with dengue were analyzed during an outbreak that occurred in Northwestern in Peru between May and June 2008. Molecular biology techniques were used to serotype the DENV, thus, firstly the viral RNA viral was extracted using Viral QIAamp RNA mini kit (Qiagen, Valencia, California, USA), then the viral cDNA fragments were reverse transcripted and amplified by means of the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and the RT-Nested PCR region techniques and finally, genetic sequencing of the viral cDNA fragments were performed using the Big Dye Terminator v.3,1 kit. Results. The 73 dengue cases presented infections by different serotypes: 34 (46.6%) by DENV-3, 29 (39.7%) by DENV-1, 4 (5.5%) by DENV-4, and 6 (8.2%) concurrent infections by DENV-1 and DENV-3. The most frequent clinical manifestations observed among dengue patients were fever and headache (100%), myalgia (94.5%), ocular pain (83.6%), arthralgia (78.1%), shivers (63.0%), nausea/vomiting (38.4%), positive tourniquet test (30.1%), and rash (20.5%). All patients with concurrent infections presented light clinical course of dengue fever (Df) except one patient who had moderate hemorrhagic manifestations. Conclusion. This is the first Peruvian report of patients with concurrent infections of two DENV serotypes without severe clinical manifestations. [ABSTRACT FROM AUTHOR]

Objetivo: identificar corredores y presencia de estacionalidad en la circulación de los serotipos de dengue se realizó mapas de calor de los serotipos de dengue según departamento año y mes. Métodos: se realizó un estudio descriptivo de la distribución geográfica y temporal de las muestras confirmadas de dengue por los métodos de RT-PCR y Aislamiento y Tipificación Viral registradas en el NETLAB entre los años 2010 y 2017 (hasta el primero de octubre) por el Laboratorio Nacional de Referencia del INS. Las bases de datos exportadas del sistema en formato Excel fueron consolidadas en el formato rda del software estadístico R versión 3.3.0, donde se realizó la limpieza y estructuración de la base de datos para efectos del estudio. Resultados: entre los años 2010 y 2017 se presenta un cambio en la distribución geográfica y temporal de los serotipos de dengue. Del 2010 al 2013 los serotipos 1 y 4 circulaban en la mayoría departamentos, a partir del año 2014 la propagación del serotipo 1 se acentuó en los departamentos del norte y en Madre de Dios solo el serotipo 4. Desde el 2011, el serotipo 2 se propagó en diferentes departamentos, desplazando al serotipo 1 hasta la actualidad. El patrón de propagación en los serotipos 2 y 3 es el mismo en los departamentos de la costa norte a partir del año 2014. Conclusiones: la distribución geográfica y temporal es una estrategia que identifica y dimensiona la circulación de los serotipos de dengue en los departamentos, y orienta la estrategia de control en zonas con la presencia de más de un serotipo circulante. [ABSTRACT FROM AUTHOR]

Our objective was to determine the genotype of the dengue virus type 2 (DENV-2) that circulated in the Amazon region of Peru between November 2010 and January 2011. We analyzed eight samples collected during dengue surveillance activities in the cities of Iquitos, yurimaguas, Trujillo, Tarapoto and Lima between November 2010 and January 2011 that were sent to Insitituto Nacional de Salud. The viruses were isolated in C6/36 HT cell line. Viral RNA was extracted and the serotype (RT - PCR multiplex) and genotype (RT-Nested PCR of the region E/NS1) were determined. finally, the E/ NS1 amplicons were sequenced and analyzed by phylogeny. The phylogenetic analysis revealed the introduction of a different lineage which entered in Peru by the end of 2010. These isolates found in Iquitos and other cities in Peru are closely related to DENV-2 isolates that circulated in Brazil during 2007 and 2008, associated with severe dengue cases and deaths. In conclusion, we detected the introduction of a different lineage of DENV-2 America / Asia genotype in Peru that could be associated with the presence of more severe cases. [ABSTRACT FROM AUTHOR]