5 results on '"Wen-Hao Wu"'
Search Results
2. Thymic stromal lymphopoietin-activated invariant natural killer T cells trigger an innate allergic immune response in atopic dermatitis
- Author
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Ji Yeon Noh, Kwang Hoon Lee, Sang Ho Oh, Chang Ook Park, Byung Gi Bae, Yeon Sook Kwon, Hee Jung Kim, and Wen Hao Wu
- Subjects
Adult ,Male ,Allergy ,Thymic stromal lymphopoietin ,Immunology ,Population ,Cell Communication ,Biology ,Lymphocyte Activation ,Dermatitis, Atopic ,Immune system ,Thymic Stromal Lymphopoietin ,medicine ,Immunology and Allergy ,Humans ,Receptors, Cytokine ,education ,Skin ,education.field_of_study ,Innate immune system ,T-cell receptor ,Dendritic cell ,Dendritic Cells ,Natural killer T cell ,medicine.disease ,Flow Cytometry ,Immunohistochemistry ,Immunity, Innate ,Gene Expression Regulation ,Cytokines ,Natural Killer T-Cells ,Female - Abstract
Although invariant natural killer T (iNKT) cells have been shown to play a critical role in the pathogenesis of asthma, the role of iNKT cells in atopic dermatitis (AD) has not been well evaluated.We investigated whether iNKT cells in patients with AD increased and whether iNKT cells were activated by thymic stromal lymphopoietin (TSLP), which is highly expressed in keratinocytes of AD.We assessed the population of iNKT cells in PBMCs of patients with AD and healthy controls (HCs) using flow cytometry. Immunohistochemistry was used to evaluate iNKT cells and TSLP expression in AD and HC skin. We also evaluated whether iNKT cells expressed the TSLP receptor, the effects of TSLP on iNKT cells, and iNKT cell-dendritic cell interactions in a TSLP-rich environment.There were more iNKT cells among PBMCs of patients with moderate to severe AD than mild AD (P.05) and HC (P.001). The number of iNKT cells was significantly larger in severe AD skin lesions than in mild (P.001) or moderate AD skin lesions (P.05). TSLP expression increased in lesional skin (P.001) but not in the sera of patients with AD (P = .729) compared with HC. iNKT cells expressed TSLP receptor protein and mRNA. TSLP directly activated iNKT cells to secrete IL-4 and IL-13, and the concurrent addition of dendritic cells further activated IFN-gamma expression.Increased iNKT cells activated by TSLP, especially in patients with severe AD, might play an essential role in the innate allergic immune response in AD.
- Published
- 2009
3. Corticotropin-releasing factor decreases IL-18 in the monocyte-derived dendritic cell
- Author
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Ju Hee Lee, Yeon Sook Kwon, Nam Soo Chang, Chang Ook Park, Wen Hao Wu, Hee Jung Lee, Sang Ho Oh, Min Geol Lee, and Kwang Hoon Lee
- Subjects
endocrine system ,medicine.medical_specialty ,Allergy ,Corticotropin-Releasing Hormone ,medicine.medical_treatment ,Dermatology ,Biochemistry ,Receptors, Corticotropin-Releasing Hormone ,Monocytes ,Dermatitis, Atopic ,Internal medicine ,medicine ,Humans ,Protein Isoforms ,RNA, Messenger ,Antigen-presenting cell ,Receptor ,Molecular Biology ,Cells, Cultured ,Chemokine CCL22 ,business.industry ,Interleukin-6 ,Monocyte ,CCL18 ,Interleukin-18 ,Dendritic cell ,Dendritic Cells ,medicine.disease ,Endocrinology ,Cytokine ,medicine.anatomical_structure ,Case-Control Studies ,Chemokines, CC ,Immunology ,Chemokine CCL17 ,business ,hormones, hormone substitutes, and hormone antagonists ,CCL22 - Abstract
Recent evidence suggests that crosstalk between mast cells, nerves and keratinocytes might be involved in the exacerbation of inflammatory conditions by stress, but the mechanism by which this occurs remains unclear. Corticotropin-releasing factor (CRF), which activates the hypothalamo-pituitary-adrenal (HPA) axis under stress, also has pro-inflammatory peripheral effects. However, there have been no reports about CRF receptor expression and the functional role of CRF in the dendritic cell (DC), which is considered to be the link between allergen uptake and the clinical manifestations of allergic diseases, such as atopic dermatitis. The purpose of this study was to investigate the expression of CRF receptors and the functional role of CRF in the monocyte-derived DC (MoDC) of atopic dermatitis patients and non-atopic healthy controls. In this study, mRNAs for CRF-R1alpha and 1beta, as well as the CRF-R1 protein, were detected in MoDCs. CRF-R2alpha (but not R2beta or R2gamma) mRNA and the CRF-R2 protein were present in MoDCs. Exposure of DCs to CRF resulted in a decrease of IL-18 in both atopic dermatitis patients and non-atopic healthy controls. However, CRF did not alter the expression of IL-6, CCL17, CCL18, and CCL22. Therefore, our results demonstrate that CRF could modulate immune responses by acting directly upon DCs.
- Published
- 2008
4. Corticotropin-releasing factor decreases IL-18 in the monocyte-derived dendritic cell.
- Author
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Hee Jung Lee, Yeon Sook Kwon, Chang Ook Park, Sang Ho Oh, Ju Hee Lee, Wen Hao Wu, Nam Soo Chang, Min-Geol Lee, and Kwang Hoon Lee
- Subjects
CORTICOTROPIN releasing hormone ,DENDRITIC cells ,MAST cells ,KERATINOCYTES ,IMMUNE response ,MESSENGER RNA ,ALLERGIES - Abstract
Recent evidence suggests that crosstalk between mast cells, nerves and keratinocytes might be involved in the exacerbation of inflammatory conditions by stress, but the mechanism by which this occurs remains unclear. Corticotropin-releasing factor (CRF), which activates the hypothalamo-pituitary-adrenal (HPA) axis under stress, also has pro-inflammatory peripheral effects. However, there have been no reports about CRF receptor expression and the functional role of CRF in the dendritic cell (DC), which is considered to be the link between allergen uptake and the clinical manifestations of allergic diseases, such as atopic dermatitis. The purpose of this study was to investigate the expression of CRF receptors and the functional role of CRF in the monocyte-derived DC (MoDC) of atopic dermatitis patients and non-atopic healthy controls. In this study, mRNAs for CRF-R1α and 1β, as well as the CRF-R1 protein, were detected in MoDCs. CRF-R2α (but not R2β or R2γ) mRNA and the CRF-R2 protein were present in MoDCs. Exposure of DCs to CRF resulted in a decrease of IL-18 in both atopic dermatitis patients and non-atopic healthy controls. However, CRF did not alter the expression of IL-6, CCL17, CCL18, and CCL22. Therefore, our results demonstrate that CRF could modulate immune responses by acting directly upon DCs. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
5. Increased expression of CC chemokine ligand 18 in extrinsic atopic dermatitis patients.
- Author
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Chang Ook Park, Hee Jung Lee, Ju Hee Lee, Wen Hao Wu, Nam Soo Chang, Liu Hua, Min-Geol Lee, and Kwang Hoon Lee
- Subjects
CHEMOKINES ,LIGANDS (Biochemistry) ,ATOPIC dermatitis ,LANGERHANS cells ,DENDRITIC cells ,MESSENGER RNA - Abstract
Background: Although most patients with atopic dermatitis (AD) show high total and allergen-specific serum immunoglobulin E (IgE) levels, a small subgroup of AD patients have normal total IgE levels and negative serum allergen-specific IgE. This subgroup has been termed ‘intrinsic’ AD (IAD) as a counterpart to ‘extrinsic’ AD (EAD). However, the difference of chemokines between IAD and EAD has not yet been evaluated. Objective: We investigated the expression of CC chemokine ligand (CCL) 17, CCL22, and CCL18 in patients with IAD and EAD, which are known to be highly expressed in AD patients. Methods: We assessed the protein levels of these chemokines in peripheral blood mononuclear cells (PBMCs), sera and lesional skins. We also evaluated CCL18 mRNA levels in monocytes, Langerhans cell (LC)-like dendritic cells (DCs) and inflammatory dendritic epidermal cell (IDEC)-like DCs from both types of AD patients. Results: There were no significant differences in CCL17 and CCL22 expression in PBMCs, sera and lesional skins of patients with IAD and EAD. CCL18 expression did not differ in PBMCs, sera and LC-like DCs from the two subgroups, but strong CCL18 expression was observed in lesional skins and IDEC-like DCs in patients with EAD. Lastly, serum CCL18 levels significantly decreased after immunotherapy. Conclusion: This study suggests that the chemokine micromilieu, especially the level of CCL18, is different between EAD and IAD patients. High FcεRI surface-expressing DCs, such as IDEC, were the major source of CCL18, and produced a prominent CCL18 microenvironment in EAD patients compared with IAD patients. [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
- View/download PDF
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