1. Controlled density glycodendron microarrays for studying carbohydrate-lectin interactions.
- Author
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Di Maio A, Cioce A, Achilli S, Thépaut M, Vivès C, Fieschi F, Rojo J, and Reichardt NC
- Subjects
- Humans, Carbohydrates chemistry, Microarray Analysis, Azides chemistry, Surface Properties, Polysaccharides chemistry, Polysaccharides metabolism, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Alkynes chemistry, Tin Compounds chemistry, Lectins chemistry, Lectins metabolism, Dendrimers chemistry
- Abstract
Glycodendron microarrays with defined valency have been constructed by on-chip synthesis on hydrophobic indium tin oxide (ITO) coated glass slides and employed in lectin-carbohydrate binding studies with several plant and human lectins. Glycodendrons presenting sugar epitopes at different valencies were prepared by spotwise strain-promoted azide-alkyne cycloaddition (SPAAC) between immobilised cyclooctyne dendrons and azide functionalised glycans. The non-covalent immobilisation of dendrons on the ITO surface by hydrophobic interaction allowed us to study dendron surface density and SPAAC conversion rate by in situ MALDI-TOF MS analysis. By diluting the dendron surface density we could study how the carbohydrate-lectin interactions became exclusively dependant on the valency of the immobilised glycodendron.
- Published
- 2021
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