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In this work, biotransformations of dehydroepiandrosterone (DHEA) 1 by Ulocladium chartarum MRC 72584, Cladosporium sphaerospermum MRC 70266 and Cladosporium cladosporioides MRC 70282 have been reported. U. chartarum MRC 72584 mainly hydroxylated 1 at C-7α and C-7β, accompanied by a minor hydroxylation at C-4β, a minor epoxidation from the β-face and a minor oxidation at C-7 subsequent to its hydroxylations. 3β,7β-Dihydroxy-5β,6β-epoxyandrostan-17-one 6, 3β,4β,7α-trihydroxyandrost-5-en-17-one 7 and 3β,4β,7β-trihydroxyandrost-5-en-17-one 8 from this incubation were identified as new metabolites. C. sphaerospermum MRC 70266 converted some of 1 into a 3-keto-4-ene steroid and then hydroxylated at C-6α, C-6β and C-7α, accompanied a minor 5α-reduction and a minor oxidation at C-6 following its hydroxylations. C. sphaerospermum MRC 70266 also hydroxylated some of 1 at C-7α and C-7β. C. cladosporioides MRC 70282 converted almost half of 1 into a 3-keto-4-ene steroid and then hydroxylated at C-6α and C-6β. C. cladosporioides MRC 70282 also reduced some of 1 at C-17. [ABSTRACT FROM AUTHOR]

The biotransformation of dehydroepiandrosterone, progesterone and pregnenolone by Aspergillus candidus MRC 200634 for 5 days is reported. Dehydroepiandrosterone was hydroxylated at C-6β, C-7β, C-7α, C-11α and C-15α. The 17-ketone was reduced to the 17β -alcohol whilst ring A of dehydroepiandrosterone was oxidised to a 4-en-3-one moiety. Progesterone was mainly hydroxylated at C-11α and C-15β and to a lesser extent at C-14α. Pregnenolone gave the same metabolites. [ABSTRACT FROM AUTHOR]

In this work, incubations of testosterone, dehydroepiandrosterone and pregnenolone with Mucor hiemalis MRC 70325 have been reported. Incubation of testosterone afforded androst-4-en-3,17-dione (3%), 14α-hydroxyandrost-4-en-3,17-dione (9%), 6β-hydroxyandrost-4-en-3,17-dione (2%) and 14α,17β-dihydroxyandrost-4-en-3-one (62%). Incubation of dehy droepiandrosterone afforded 3β, 17β-dihydroxyandrost-5-ene (6%) and 3β, 7α-dihydroxyandrost-5-en-17-one (72%). Incubation of pregnenolone afforded 3β, 14α-dihydroxypregn-5-en-7,20-dione (3%), 3β, 7α-dihydroxypregn-5-en-20-one (64%) and 3β, 7α, 11α-trihydroxypregn-5-en-20-one (11%). 3β, 14α-Dihydroxypregn-5-en-7,20-dione was identified as a new metabolite. [ABSTRACT FROM AUTHOR]