1. New insight into molecular mechanisms of different polyphenols affecting Sirtuin 3 deacetylation activity.
- Author
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Wei, Yulong, Zhang, Yiman, Wang, Ziyuan, Yang, Zihui, Wang, Zongwei, Hao, Yiming, Li, Genying, Gao, Fei, Ye, Gaoqi, Wang, Jing, and Liu, Jie
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DEACETYLATION , *PLANT polyphenols , *PHENOLS , *PEPTIDES , *POLYPHENOLS , *QUERCETIN - Abstract
Multiple phenolic substances have been shown to promote SIRT3 expression, however, few studies have focused on the effects of these phenolics on SIRT3 enzyme activity. This study constructed a variety of reaction systems to elucidate the mechanisms by which different polyphenols affect SIRT3 enzyme activity. The results showed that acP53 317 – 320 was the most suitable substrate among the five acetylated peptide substrates (K cat / K m = 74.85 ± 1.86 M−1•s−1). All the phenolic compounds involved in the experiment inhibited the enzymatic activity of SIRT3, and the lowest IC 50 among them was quercetin (0.12 ± 0.01 mM) and the highest was piceatannol (1.29 ± 0.08 mM). Their inhibition types were mainly competitive and mixed. In addition, piceatannol was found to be a natural SIRT3 agonist by enzyme kinetic analysis and validation of deacetylation efficiency. This study will provide a useful reference for polyphenol modulation of SIRT3 dosage, as well as the development and application of polyphenol-based SIRT3 activators and agonists. • The IC 50 for inhibition of SIRT3 enzyme activity by different polyphenols was detected. • Interaction mechanisms between different polyphenols and SIRT3 were analyzed. • Mechanistic analysis of piceatannol enhancing the enzymatic activity of SIRT3. • A strategy for agonist or inhibitor screening of SIRT3 was constructed. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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