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2 results on '"Pena, Gisel"'

1. Evaluation of serial crystallographic structure determination within megahertz pulse trains

Author

Yefanov, Oleksandr, Oberth��r, Dominik, Bean, Richard, Wiedorn, Max Oliver, Kno��ka, Juraj, Pena, Gisel, Awel, Salah, Gumprecht, Lars, Domaracky, Martin, Sarrou, Iosifina, Xavier, Paulrajpillai Lourdu, Metz, Markus, Bajt, Sa��a, Mariani, Valerio, Gevorkov, Yaroslav, White, Thomas A., Tolstikova, Aleksandra, Villanueva-Perez, Pablo, Seuring, Carolin, Aplin, Steven, Estillore, Armando D., K��pper, Jochen, Klyuev, Alexander, Kuhn, Manuela, Laurus, Torsten, Graafsma, Heinz, Monteiro, Diana C.F., Trebbin, Martin, Maia, Filipe R. N. C., Cruz-Mazo, Francisco, Ga����n-Calvo, Alfonso M., Heymann, Michael, Darmanin, Connie, Abbey, Brian, Schmidt, Marius, Fromme, Petra, Giewekemeyer, Klaus, Sikorski, Marcin, Graceffa, Rita, Vagovic, Patrik, Kluyver, Thomas, Bergemann, Martin, Fangohr, Hans, Sztuk-Dambietz, Jolanta, Hauf, Steffen, Raab, Natascha, Bondar, Valerii, Mancuso, Adrian P., Chapman, Henry N., and Barty, Anton

Subjects
Fysikalisk kemi, ARTICLES, lcsh:QD901-999, 004: Informatik, Acceleratorfysik och instrumentering, lcsh:Crystallography, ddc:500, Biological Systems, ddc:004, Informatik [004], Accelerator Physics and Instrumentation, Physical Chemistry
Abstract

Structural dynamics 6(6), 064702 - (2019). doi:10.1063/1.5124387, Published by AIP Publishing LLC, Melville, NY

Published
2019
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2. Megahertz serial crystallography

Author

Wiedorn, Max O., Oberthuer, Dominik, Bean, Richard, Schubert, Robin, Werner, Nadine, Abbey, Brian, Aepfelbacher, Martin, Adriano, Luigi, Allahgholi, Aschkan, Al-Qudami, Nasser, Andreasson, Jakob, Aplin, Steve, Awel, Salah, Ayyer, Kartik, Bajt, Sasa, Barak, Imrich, Bari, Sadia, Bielecki, Johan, Botha, Sabine, Boukhelef, Djelloul, Brehm, Wolfgang, Brockhauser, Sandor, Cheviakov, Igor, Coleman, Matthew A., Cruz-Mazo, Francisco, Danilevski, Cyril, Darmanin, Connie, Doak, R. Bruce, Domaracky, Martin, Doerner, Katerina, Du, Yang, Fangohr, Hans, Fleckenstein, Holger, Frank, Matthias, Fromme, Petra, Ganan-Calvo, Alfonso M., Gevorkov, Yaroslav, Giewekemeyer, Klaus, Ginn, Helen Mary, Graafsma, Heinz, Graceffa, Rita, Greiffenberg, Dominic, Gumprecht, Lars, Goettlicher, Peter, Hajdu, Janos, Hauf, Steffen, Heymann, Michael, Holmes, Susannah, Horke, Daniel A., Hunter, Mark S., Imlau, Siegfried, Kaukher, Alexander, Kim, Yoonhee, Klyuev, Alexander, Knoska, Juraj, Kobe, Bostjan, Kuhn, Manuela, Kupitz, Christopher, Kueper, Jochen, Lahey-Rudolph, Janine Mia, Laurus, Torsten, Le Cong, Karoline, Letrun, Romain, Xavier, P. Lourdu, Maia, Luis, Maia, Filipe R. N. C., Mariani, Valerio, Messerschmidt, Marc, Metz, Markus, Mezza, Davide, Michelat, Thomas, Mills, Grant, Monteiro, Diana C. F., Morgan, Andrew, Muhlig, Kerstin, Munke, Anna, Muennich, Astrid, Nette, Julia, Nugent, Keith A., Nuguid, Theresa, Orville, Allen M., Pandey, Suraj, Pena, Gisel, Villanueva-Perez, Pablo, Poehlsen, Jennifer, Previtali, Gianpietro, Redecke, Lars, Riekehr, Winnie Maria, Rohde, Holger, Round, Adam, Safenreiter, Tatiana, Sarrou, Iosifina, Sato, Tokushi, Schmidt, Marius, Schmitt, Bernd, Schoenherr, Robert, Schulz, Joachim, Sellberg, Jonas A., Seibert, M. Marvin, Seuring, Carolin, Shelby, Megan L., Shoeman, Robert L., Sikorski, Marcin, Silenzi, Alessandro, Stan, Claudiu A., Shi, Xintian, Stern, Stephan, Sztuk-Dambietz, Jola, Szuba, Janusz, Tolstikova, Aleksandra, Trebbin, Martin, Trunk, Ulrich, Vagovic, Patrik, Ve, Thomas, Weinhausen, Britta, White, Thomas A., Wrona, Krzysztof, Xu, Chen, Yefanov, Oleksandr, Zatsepin, Nadia, Zhang, Jiaguo, Perbandt, Markus, Mancuso, Adrian P., Betzel, Christian, Chapman, Henry, and Barty, Anton

Subjects
Fysikalisk kemi, Science, Acceleratorfysik och instrumentering, Electrical Engineering, Electronic Engineering, Information Engineering, Accelerator Physics and Instrumentation, Physical Chemistry, Article, lcsh:Q, ddc:500, Elektroteknik och elektronik, lcsh:Science, ddc:600, Technik [600]
Abstract

The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source., The new European X-Ray Free-Electron Laser (EuXFEL) is the first XFEL that generates X-ray pulses with a megahertz inter-pulse spacing. Here the authors demonstrate that high-quality and damage-free protein structures can be obtained with the currently available 1.1 MHz repetition rate pulses using lysozyme as a test case and furthermore present a β-lactamase structure.

Published
2018

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