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This paper presents an enhanced method to locate and quantify damage in beam-like structures using changes in deflections estimated from modal flexibility (MF) matrices. The method is developed from explicit relationship between a series of MF-based deflection change vectors and the damage characteristics. Based on this, three damage locating criteria are defined and used to detect and locate damage. Once the damage is located, its severity is estimated conveniently from a closed-form function. The capability of the proposed method is examined through numerical and experimental verifications on a steel beam model. The result shows that the method accurately locates and quantifies damage under various scenarios using a few modes of vibration, with satisfactory or even better results compared to those obtained from traditional static deflection-based method. The performance of the proposed method is also compared with three well-known vibration-based damage detection methods using changes in MF and modal strain energy. It is found that the proposed method outperformed the other three methods, especially for multiple damage cases. As beams can represent various structural components, the proposed method provides a promising damage identification tool targeting the application to real-life structures. [ABSTRACT FROM AUTHOR]