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Cull cows from dairy farms in the United States account for 10% of the beef market; however, few studies have evaluated the effects of crossbreeding on reasons for disposal and cull value of dairy cows. The objective of the study was to compare reasons for disposal and cull cow value of Holstein and crossbred cows (n = 1,292) in an experimental dairy herd at the University of Minnesota West Central Research and Outreach Center (Morris, MN). Cows were Holstein (n = 272), 1964 genetic control Holstein (n = 161), 3-breed crossbred cows (n = 538) composed of the Montbéliarde, Viking Red, and Holstein (MVH) breeds, and 3-breed crossbred cows (n = 321) composed of the Normande, Jersey, and Viking Red (NJV) breeds. The 1964 genetic control Holstein cows originated from a design initiated at the University of Minnesota for comparison of the 1964 Holstein and contemporary Holstein cows selected for production across time. Records spanned from January 2010 to December 2023. Reasons for disposal were recorded in PCDart Herd Management Software, and cull value and body weight were obtained from receipts from livestock cull markets. For all cows, the primary reasons for disposal were as follows: reproduction (44.4%), mastitis (18%), other reasons (14.6%), death (10.3%), dairy purposes (8.4%), and low production (4.3%). Independent variables for statistical analysis of cull value were the fixed effects of body weight at time of culling, DIM at culling (0–49 DIM, 50–99 DIM, 100–149 DIM, 150–199 DIM, 200–249 DIM, 250–299 DIM, 300–350 DIM, and >350 DIM), year (2010–2023), season (spring, summer, autumn, winter), parity (1, 2, 3, 4, 5+), primary reason for culling, breed group, and sire breed group nested within breed group. Least squares means for body weight at culling was 558 kg for Holsteins, 543 kg for 1964 Holsteins, 551 kg for MVH crossbred cows, and 499 kg for NJV crossbred cows. For the analysis across time, 2014 had the highest cull value ($1,126.98), and 2020 had the lowest gross cull value ($515.21). Cows culled at greater than 300 DIM had the highest cull value and cows culled at less than 50 DIM had the lowest cull value. Cows culled during the spring and summer had higher cull value compared with cows culled during the autumn and winter. The Holstein cows ($730.04) had lower gross value compared with 1964 Holsteins ($804.38) cows and MVH ($767.39) cows. The NJV ($771.39) cows were not different from crossbreds sired by Montbéliarde, Viking Red, and Holstein bulls. Results for comparisons of breed group are from one experimental herd, so inferences to the wider dairy cow population should be undertaken with caution. In summary, dairy producers may receive greater cull value from crossbred cows compared with Holstein cows. The list of standard abbreviations for JDS is available at adsa.org/jds-abbreviations-24. Nonstandard abbreviations are available in the Notes. [ABSTRACT FROM AUTHOR]