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Simple Summary: An investigation of poor aerobic stability in high-moisture total mixed rations (TMRs) for livestock feed was conducted in this study. TMR quality factors were discovered, and prospective approaches to increase its fermentation properties and overall stability were investigated. Using infrared thermography (IRT) measurements, it was suggested that dairy cow feeding methods can be optimized in the field by identifying portions with a higher center temperature and maximum temperature difference values. According to the findings, there is a significant potential for IRT to be used in feed management and preservation processes in the future, resulting in higher levels of productivity for livestock. A major objective of this study is to identify factors influencing the quality of high-moisture total mixed rations (TMRs) for livestock feed and explore possible manipulations that can enhance their fermentation characteristics and stability in order to address the problem of poor aerobic stability. Therefore, the current study utilized infrared thermography (IRT) to assess the aerobic stability of water-added TMRs in the feed bunker. By manipulating the moisture content of freshly prepared TMRs at four different levels through water addition and subjecting it to storage at two consistent temperatures, significant correlations between IRT values (center temperature (CT) and maximum temperature difference (MTD)) and key parameters such as lactic acid bacteria, water-soluble carbohydrates, and TMR pH were established. The first and second principal components together accounted for 44.3% of the variation, with the first component's load influenced by IRT parameters, fermentation characteristics, and air exposure times, while the second component's load was influenced by dry matter content and lactic acid concentration. The results of these studies indicate the possibility that feeding methods can be optimized by identifying portions with higher CT or MTD data using IRT measurements just before feeding dairy cows in the field. As a result, increasing the use of IRT in feed management and preservation processes is projected to have a positive impact on animal productivity in the future. [ABSTRACT FROM AUTHOR]

This research aims to evaluate the potential of lavender meal (LM) and lavender essential oil (LEO) to mitigate methane emissions by dairy cows. Locally grown lavender was collected fresh for this purpose, and its oil was extracted using the cold-press method. The resultant LEO and LM and whole lavender (WL) were added to dairy cow concentrate feed at 0%, 0.05%, and 0.10%, and their effects on vitro gas production values and gas concentrations were subsequently assessed. Out of the 30 bioactive compounds isolated from LEO, linalool and linalyl acetate were the most common—accounting for 70.4% of the total. The lavender dose had a significant influence on gas production for up to 12 h. No significant variations were found across the lavender forms when gas kinetics, in vitro degradability, and predicted energy values were compared. The addition of WL to the concentrate feed of dairy cows produced the greatest quantities of methane, carbon dioxide, and hydrogen sulfide, whereas LEO resulted in the lowest values. In contrast, no significant difference in ammonia content was found across the various lavender forms added into dairy cow concentrate feed. The results of this research suggest that adding 0.05–0.10% LM and LEO to concentrate feed may decrease greenhouse gas emissions from dairy cows. [ABSTRACT FROM AUTHOR]

Modifying the chemical composition of a diet can be a good strategy for reducing methane emission in the rumen. However, this strategy can have adverse effects on the ruminal microbial flora. The aim of our study was to reduce methane without disturbing ruminal function by stimulating the growth and propagation of methanotrophs. In this study, we randomly divided twenty multiparous Holstein dairy cows into 4 groups in a 2×2 factorial design with two forage levels (40% and 60%) and two nitrate supplementation levels (3.5% and zero). We examined the effect of experimental diets on cow performance, ruminal fermentation, blood metabolites and changes of ruminal microbial flora throughout the experimental period (45-day). Additionally, in vitro methane emission was evaluated. Animals fed diet with 60% forage had greater dry matter intake (DMI) and milk fat content, but lower lactose and milk urea content compared with those fed 40% forage diet. Moreover, nitrate supplementation had no significant effect on DMI and milk yield. Furthermore, the interactions showed that nitrate reduces DMI and milk fat independently of forage levels. Our findings showed that nitrate can increase ammonia concentration, pH, nitrite, and acetate while reducing the total volatile fatty acids concentration, propionate, and butyrate in the rumen. With increasing nitrate, methane emission was considerably decreased possibly due to the stimulated growth of Fibrobacteria, Proteobacteria, type II Methanotrophs, and Methanoperedense nitroreducens, especially with high forage level. Overall, nitrate supplementation could potentially increase methane oxidizing microorganisms without adversely affecting cattle performance. [ABSTRACT FROM AUTHOR]

Simple Summary: Most unsaturated fatty acids in the ruminant's diet are hydrogenated in their rumen so that the composition of the fatty acids entering the rumen and their output is significantly different. Therefore, minimizing the ruminal biohydrogenation process of unsaturated fatty acids is one of the most important issues for feed supplements manufacturers and animal nutritionists to increase the availability of these fatty acids in the intestine. In recent years, encapsulation has been used to preserve the active ingredient in livestock; it is a method used to control the release of feed additives during digestion. There is a clear need to find a more effective method by which unsaturated fatty acids present in fat supplements can be protected to bypass rumen environment and its biohydrogenation, without negative effect on digestive efficiency, and be available in lower digestive tracts. For these reasons, this study aims to evaluate the use of natural materials to encapsulate fats and their effect on in vitro fermentation and fatty acid biohydrogenation. The results indicated that the percentage of ruminal saturated fatty acids decreased by encapsulation of flaxseed oil with chitosan (14% and 7%). The percentage of oleic unsaturated fatty acid by encapsulating flaxseed oil with chitosan (14%) had a significant increase compared to the control treatment (p < 0.05). Encapsulation of flaxseed oil with chitosan (14%) reduced the unsaturated fatty acids of ruminal biohydrogenation. The aim of this study was to investigate the effect of using chitosan nanoparticles and calcium alginate in the encapsulation of flaxseed oil on the biohydrogenation of unsaturated fatty acids and in vitro fermentation. The experiments were performed in a completely randomized design with 7 treatments. The experimental treatments included: diets without oil additive (control), diet containing 7% flaxseed oil, diet containing 14% flaxseed oil, diet containing 7% oil encapsulated with 500 ppm chitosan nanocapsules, diet containing 14% flaxseed oil encapsulated with 1000 ppm chitosan nanocapsules, diet containing 7% of flaxseed oil encapsulated with 500 ppm of calcium alginate nanocapsules, diet containing 14% flaxseed oil encapsulated with 1000 ppm calcium alginate nanocapsules. The results showed that encapsulation of flaxseed oil with calcium alginate (14%) had a significant effect on gas production (p < 0.05). The treatment containing calcium alginate (14%) increased the digestibility of dry matter compared to the control treatment, but the treatments containing chitosan caused a significant reduction (p < 0.05). The results indicated that the percentage of ruminal saturated fatty acids decreased by encapsulation of flaxseed oil with chitosan (14% and 7%). The percentage of oleic unsaturated fatty acid by encapsulating flaxseed oil with chitosan (14%) had a significant increase compared to the control treatment (p < 0.05). As a result, encapsulating flaxseed oil with chitosan (14%) reduced the unsaturated fatty acids generated during ruminal biohydrogenation. [ABSTRACT FROM AUTHOR]

Nitrate may reduce the ruminal methane emission by competing methanogenesis to achieve more hydrogen. For this purpose, twenty Holstein lactating cows were examined using a 2×2 factorial design in 4 groups for 60 days with two forage levels (40% and 60%) and supplemental nitrate 0% (F40 and F60) and 3.5% (F40N and F60N) of diet dry matter (DM). Then, the effect of nitrate and forage levels on cow performance, ruminal fermentation, methane emission, and metabolic hydrogen sink were evaluated. The nitrate supplementation did not significantly affect milk yield and ECM/DMI, while milk urea nitrogen was increased. Lowest quantity of milk vitamins (A and E) was observed in nitrate groups. The nitrate supplementation increased c9-C18:1, unsaturated fatty acids, and n-6/n-3 contents of the milk. Blood parameters were affected by nitrate supplementation. Blood met-Hb concentration was increased, while blood glucose was decreased in nitrate groups. High forage and nitrate fed animals (F60N) had higher ruminal acetate and lower propionate concentration, and higher acetate+butyrate to propionate ratio than other groups. Nitrite and NH3-N concentrations were higher in the rumen of nitrate fed animals. Nitrate supplementation inhibited gas volume and methane emission without affecting volatile fatty acids at 12 and 24 h of incubation. The H2 balance, H2 production and consumption, and recovery percentage were significantly lower in F60N group. In conclusion, nitrate supplementation can be employed as an alternative strategy for improving ruminal fermentation, milk quality and methane inhibition. [ABSTRACT FROM AUTHOR]