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Your search keyword '"Jorge Luiz Raposo Junior"' showing total 3 results
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3 results on '"Jorge Luiz Raposo Junior"'

1. Genotoxic and mutagenic effects of polluted surface water in the midwestern region of Brazil using animal and plant bioassays

Author

Priscila Leocádia Rosa Dourado, Monyque Palagano da Rocha, Liriana Mara Roveda, Jorge Luiz Raposo Junior, Liliam Sílvia Cândido, Claudia Andréa Lima Cardoso, Maria Aparecida Marin Morales, Kelly Mari Pires de Oliveira, and Alexeia Barufatti Grisolia

Subjects
Allium cepa, Astyanax altiparanae, mutagenicity, cytotoxicity, genotoxicity, Genetics, QH426-470
Abstract

Abstract This study aimed to evaluate DNA damage in animal and plant cells exposed to water from the Água Boa stream (Dourados, Mato Grosso do Sul, Brazil) by using bioassays, and to identify the chemical compounds in the water to determine the water quality in the area. Through the cytotoxicity bioassay with Allium cepa, using micronucleus test, and comet assay, using Astyanax altiparanae fish, the results indicated that biological samples were genetically altered. Micronuclei were observed in erythrocytes of A. altiparanae after exposure to water from locations close to industrial waste discharge. The highest DNA damage observed with the comet assay in fish occurred with the exposure to water from locations where the presence of metals (Cu, Pb, Cd, Ni) was high, indicating the possibility of genotoxic effects of these compounds. Thus, these results reinforce the importance of conducting genotoxicity tests for developing management plans to improve water quality, and indicate the need for waste management before domestic and industrial effluents are released into the rivers and streams.

Published
2016
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2. Genotoxic and mutagenic effects of polluted surface water in the midwestern region of Brazil using animal and plant bioassays

Author

Priscila Leocádia Rosa Dourado, Monyque Palagano da Rocha, Liriana Mara Roveda, Jorge Luiz Raposo Junior, Liliam Sílvia Cândido, Claudia Andréa Lima Cardoso, Maria Aparecida Marin Morales, Kelly Mari Pires de Oliveira, and Alexeia Barufatti Grisolia

Subjects
Allium cepa, Astyanax altiparanae, mutagenicity, cytotoxicity, genotoxicity, Genetics, QH426-470
Abstract

Abstract This study aimed to evaluate DNA damage in animal and plant cells exposed to water from the Água Boa stream (Dourados, Mato Grosso do Sul, Brazil) by using bioassays, and to identify the chemical compounds in the water to determine the water quality in the area. Through the cytotoxicity bioassay with Allium cepa, using micronucleus test, and comet assay, using Astyanax altiparanae fish, the results indicated that biological samples were genetically altered. Micronuclei were observed in erythrocytes of A. altiparanae after exposure to water from locations close to industrial waste discharge. The highest DNA damage observed with the comet assay in fish occurred with the exposure to water from locations where the presence of metals (Cu, Pb, Cd, Ni) was high, indicating the possibility of genotoxic effects of these compounds. Thus, these results reinforce the importance of conducting genotoxicity tests for developing management plans to improve water quality, and indicate the need for waste management before domestic and industrial effluents are released into the rivers and streams.

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3. Cytotoxicity, Genotoxicity and Mutagenicity of Aluminum, Manganese and Lead in Meristematic Cells of Root Allium cepa

Author

Jorge Luiz Raposo Junior, Luiza Flavia Veiga Franscisco, Alexeia Barufatti Grisolia, Lucilene Finoto Viana, Hélina dos Santos Nascimento, and Bruno do Amaral Crispim

Subjects
Mitotic index, Materials Science (miscellaneous), General Chemical Engineering, Science, metals, medicine.disease_cause, average root growth, ecotoxicology, medicine, Radicle, Bioassay, Ecotoxicology, Food science, Cytotoxicity, QD1-999, biology, Chemistry, 0402 animal and dairy science, 04 agricultural and veterinary sciences, General Chemistry, biology.organism_classification, 040201 dairy & animal science, germination, Germination, 040103 agronomy & agriculture, 0401 agriculture, forestry, and fisheries, Allium, Genotoxicity
Abstract

Metals are chemical contaminants that are not normally biodegradable and accumulate in living organisms. In addition, they may present toxic effects to plants and affect human health causing mutations and adn damage of exposed organisms. The objective of this study was to evaluate the cytotoxicity, genotoxicity and mutagenicity of the aluminum, manganese and lead using A.cepa test. Solutions in three concentrations with the metals and controls were prepared for the genetic analyzes. The seeds of A. cepa were exposed to germination, and evaluation of cytotoxicity, genotoxicity and mutagenicity. The results showed that the concentrations of the metals did not interfere in the growth of the radicle, but the increase in the concentration of the metals was directly proportional to the increase of the number of seeds germinated. For the mitotic index it was observed that the increase in Al concentration provided the increase of cells in cell division, but for Mn and Pb there was reduction. The metals at the concentrations evaluated did not present genotoxic and mutagenic potential for meristematic cells of A. cepa. The A. cepa test it showed to be effective for performed analyzes, however, further studies are needed using other biological assays. DOI: http://dx.doi.org/10.17807/orbital.v10i1.1037

Published
2018

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