1. Natural Killer Cells from Allergic Donors Are Defective in Their Response to CCL18 Chemokine
- Author
-
Amniai, Latiffa, Ple, Coline, Barrier, Mathieu, de Nadai, Patricia, Marquillies, Philippe, Vorng, Han, Chenivesse, Cécile, Tsicopoulos, Anne, Duez, Catherine, Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), and CHU Lille
- Subjects
Cytotoxicity, Immunologic ,[SDV]Life Sciences [q-bio] ,Chemotaxis ,natural killer cell ,migration ,allergy ,Article ,Killer Cells, Natural ,lcsh:Chemistry ,lcsh:Biology (General) ,lcsh:QD1-999 ,GTP-Binding Proteins ,Case-Control Studies ,Chemokines, CC ,Hypersensitivity ,CCL18 ,Cytokines ,Humans ,cytotoxicity ,lcsh:QH301-705.5 ,Biomarkers - Abstract
International audience; Natural killer (NK) cells were originally described as cytolytic effector cells, but since then have been recognized to possess regulatory functions on immune responses. Chemokines locate NK cells throughout the body in homeostatic and pathological conditions. They may also directly stimulate immune cells. CCL18 is a constitutive and inducible chemokine involved in allergic diseases. The aim of this study was to evaluate CCL18’s effect on NK cells from allergic and nonallergic donors in terms of both chemotactic and immune effects. Results showed that CCL18 was able to induce migration of NK cells from nonallergic donors in a G-protein-dependent manner, suggesting the involvement of a classical chemokine receptor from the family of seven-transmembrane domain G-protein-coupled receptors. In contrast, NK cells from allergic patients were unresponsive. Similarly, CCL18 was able to induce NK cell cytotoxicity only in nonallergic subjects. Purified NK cells did not express CCR8, one of the receptors described to be involved in CCL18 functions. Finally, the defect in CCL18 response by NK cells from allergic patients was unrelated to a defect in CCL18 binding to NK cells. Overall, our results suggest that some NK cell functions may be defective in allergic diseases.
- Published
- 2021