1. Stochastic modeling reveals how motor protein and filament properties affect intermediate filament transport
- Author
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Sandrine Etienne-Manneville, Stéphanie Portet, Cécile Leduc, J. C. Dallon, Brigham Young University (BYU), Polarité cellulaire, Migration et Cancer - Cell Polarity, Migration and Cancer, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), University of Manitoba [Winnipeg], This work was supported by: EPSRC grant numbers EP/K032208/1 and EP/R014604/1 and by La Ligue contre le cancer and the Institut Pasteur., The authors would like to thank the Isaac Newton Institute for Mathematical Sciences for support and hospitality during the programme 'Coupling Geometric PDE’s with Physics for Cell Morphology, Motility and Pattern Formation' when the work on this paper was undertaken., Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), and ANR-16-CE13-0019,SiFi2Net,Filaments intermédiaires: du filament unique au réseau(2016)
- Subjects
0301 basic medicine ,Statistics and Probability ,Materials science ,[SDV]Life Sciences [q-bio] ,Dynein ,Biological Transport, Active ,Kinesins ,Catch bond ,macromolecular substances ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,Microtubules ,Models, Biological ,General Biochemistry, Genetics and Molecular Biology ,Quantitative Biology::Cell Behavior ,Protein filament ,Motor protein ,Quantitative Biology::Subcellular Processes ,03 medical and health sciences ,0302 clinical medicine ,Molecular motor ,Vimentin ,Intermediate filaments ,Intermediate filament ,Cytoskeleton ,Stochastic Processes ,General Immunology and Microbiology ,Applied Mathematics ,Dyneins ,Molecular motors ,General Medicine ,Kinesin ,Stochastic ,030104 developmental biology ,Modeling and Simulation ,Biophysics ,General Agricultural and Biological Sciences ,030217 neurology & neurosurgery - Abstract
International audience; Intermediate filaments are a key component of the cytoskeleton. Their transport along microtubules plays an essential role in the control of the shape and structural organization of cells. To identify the key parameters responsible for the control of intermediate filament transport, we generated a model of elastic filament transport by microtubule-associated dynein and kinesin. The model is also applicable to the transport of any elastically-coupled cargoes. We investigate the effect of filament properties such as number of motor binding sites, length, and elasticity on motion of filaments. Additionally, we consider the effect of motor properties, i.e. off rates, on filament transport. When one motor has a catch bond off rate it dictates the motion, whereas when motors have the same type of off rate filaments can alternate between retrograde and anterograde motions. The elasticity of filaments optimizes the filament transport and the coordination of motors along the length of the filament.
- Published
- 2019