1. Immunomodulating effects of CKBM on the cytokine production in peripheral blood mononuclear cells (PBMCs) from healthy volunteers.
- Author
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Chan BP, Yuen WF, Lee WH, Wong SN, Chung TY, Wu YJ, and Pang SF
- Subjects
- Adult, Cell Survival drug effects, Dose-Response Relationship, Drug, Female, Humans, Immunologic Factors administration & dosage, Immunologic Factors toxicity, In Vitro Techniques, Interferon-gamma biosynthesis, Interleukin-6 biosynthesis, Male, Middle Aged, Plant Extracts administration & dosage, Plant Extracts toxicity, Receptors, Tumor Necrosis Factor, Type II biosynthesis, Tumor Necrosis Factor-alpha biosynthesis, Cytokines biosynthesis, Immunologic Factors pharmacology, Leukocytes, Mononuclear drug effects, Leukocytes, Mononuclear immunology, Plant Extracts pharmacology
- Abstract
The current study investigated the immunomodulating effect of CKBM on cytokine induction in peripheral blood mononuclear cells (PBMCs) isolated from 20 healthy volunteers. Cytometric Bead Analysis (CBA) was used to study IL-2, IL-4, IL-6, IL-10, TNF-alpha and IFN-gamma. TNF-alpha and IL-6 were significantly increased in a CKBM dose- and time-dependent manner. Flow cytometry analysis showed an increased intracellular staining of IL-6 but not of TNF-alpha in CKBM treated PBMCs. In addition, MTT cell cytotoxicity assay showed that CKBM concentrations below 5% did not significantly affect the metabolic activities of PBMCs. The current study indicated that CKBM may modulate the immune response by inducing the secretions of TNF-alpha and IL-6, which are cytokine mediators of innate immunity and inflammation preparing or "priming" the body to combat diseases.
- Published
- 2004
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