1. Pre-treatment with curcumin modulates acetylcholinesterase activity and proinflammatory cytokines in rats infected with Trypanosoma evansi.
- Author
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Wolkmer P, Silva CB, Paim FC, Duarte MM, Castro V, Palma HE, França RT, Felin DV, Siqueira LC, Lopes ST, Schetinger MR, Monteiro SG, and Mazzanti CM
- Subjects
- Acetylcholinesterase metabolism, Administration, Oral, Animals, Anti-Inflammatory Agents, Non-Steroidal therapeutic use, Cholinesterase Inhibitors pharmacology, Cholinesterase Inhibitors therapeutic use, Curcumin administration & dosage, Curcumin therapeutic use, Cytokines metabolism, Disease Models, Animal, Immunologic Factors therapeutic use, Male, Parasitemia, Random Allocation, Rats, Rats, Wistar, Trypanosoma drug effects, Trypanosoma immunology, Trypanosomiasis drug therapy, Trypanosomiasis enzymology, Trypanosomiasis prevention & control, Acetylcholinesterase blood, Anti-Inflammatory Agents, Non-Steroidal pharmacology, Curcumin pharmacology, Cytokines blood, Immunologic Factors pharmacology, Trypanosomiasis immunology
- Abstract
The potent activity against Trypanosomes and health beneficial effects of curcumin (Cur) has been demonstrated in various experimental models. In this study, we evaluated the in vivo effect of Cur as trypanocide and as potential anti-inflammatory agent, through the evaluation of immunomodulatory mechanisms in rats infected with Trypanosoma evansi. Daily oral Cur was administered at doses of 0, 20 or 60mg/kg as preventive treatment (30 and 15days pre infection) and as treatment (post infection). The treatment of the groups continued until the day of euthanasia. Fifteen days after inoculation, parasitemia, plasma proinflammatory cytokines (IFN-γ, TNF-α, IL-1, IL-6), anti-inflammatory cytokines (IL-10) and blood acetylcholinesterase activity (AChE) were analyzed. Pretreatment with Cur reduced parasitemia and lethality. Cur inhibited AChE activity and improved immunological response by cytokines proinflammatory, fundamental during T. evansi infection. We found that Cur is not so important as an antitrypanosomal activity but as immunomodulator agent. These findings reveal that the preventive use of Cur stimulates anti-inflammatory mechanisms, reducing an excessive inflammatory response., (Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2013
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