1. Effect of oxygen tension on bacteria-stimulated cytokine production by fetal membranes.
- Author
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Klimova NG, Hanna N, and Peltier MR
- Subjects
- Amnion immunology, Amnion metabolism, Amnion microbiology, Chorion immunology, Chorion metabolism, Chorion microbiology, Decidua immunology, Decidua metabolism, Decidua microbiology, Extraembryonic Membranes microbiology, Female, Humans, Interleukin-10 biosynthesis, Interleukin-1beta biosynthesis, Interleukin-6 biosynthesis, Interleukin-8 biosynthesis, Pregnancy, Tissue Culture Techniques, Tumor Necrosis Factor-alpha biosynthesis, Uropathogenic Escherichia coli immunology, Uropathogenic Escherichia coli pathogenicity, Cytokines biosynthesis, Extraembryonic Membranes immunology, Extraembryonic Membranes metabolism, Oxygen metabolism
- Abstract
Aim: Tissue culture studies indicate that bacterial products stimulate the production of proinflammatory cytokines by reproductive tissues. However, most of these studies have been performed under room air conditions, supplemented with 5% CO₂. In this study, we tested whether O₂ tension affects bacteria-stimulated cytokine production by extra-placental fetal membranes., Methods: Cultures of full-thickness membranes, isolated choriodecidua, and isolated amnion were exposed to bacteria and incubated under 21% (room air) or 5% O₂ for 18 h. Cytokine concentrations in conditioned medium was quantified by immunoassay., Results: Culture under 5% O₂ increased production of interleukin (IL)-1β and tumor necrosis factor (TNF)-α, but reduced IL-10 and IL-6 production by full membranes. Isolated choriodecidua responded to 5% O₂ with increased IL-1β production and reduced IL-6 production, but had no effect on TNF-α and IL-10 production was not detected. No effect of O₂ tension on IL-1β or IL-6 production by isolated amnion was detected, however, Escherichia coli-stimulated IL-10, TNF-α and IL-8 production was enhanced by culture under 5% O₂., Conclusions: Increased oxygen tension reduces the pro-inflammatory responsiveness of cell cultures to E. coli and promotes an anti-inflammatory cytokine profile. Differential effects of O₂ tension on choriodecidua and amnion suggests a network of paracrine factors that regulate cytokine levels in response to changes in O₂ tension.
- Published
- 2013
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