1. The effect of cytokines on the proliferation and migration of bovine meniscal cells.
- Author
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Bhargava MM, Attia ET, Murrell GA, Dolan MM, Warren RF, and Hannafin JA
- Subjects
- Animals, Bone Morphogenetic Protein 2, Bone Morphogenetic Proteins pharmacology, Cattle, Cell Division, Cell Movement, Cells, Cultured, DNA biosynthesis, Dose-Response Relationship, Drug, Epidermal Growth Factor pharmacology, Fibroblast Growth Factors pharmacology, Fibroblasts cytology, Hepatocyte Growth Factor pharmacology, Humans, Insulin-Like Growth Factor I pharmacology, Insulin-Like Growth Factor II pharmacology, Interleukin-1 pharmacology, Platelet-Derived Growth Factor pharmacology, Radiopharmaceuticals, Recombinant Proteins, Transforming Growth Factor beta pharmacology, Tritium, Chondrocytes cytology, Cytokines pharmacology, Menisci, Tibial cytology
- Abstract
We determined the effect of cytokines on the proliferation and migration of cells isolated from the inner-third (white-white), middle-third (red-white), and outer-third (red-red) regions of bovine meniscus. Cells from the outer, or peripheral, region of the meniscus exhibited higher DNA synthesis in the presence of 10% serum compared with cells from the inner or central regions. Recombinant human platelet-derived growth factor-AB, hepatocyte growth factor/scatter factor, and bone morphogenic protein-2 stimulated DNA synthesis of all meniscal cells in a dose-dependent manner, with a two- to threefold maximal stimulation at 10 ng/ml. Cell migration was also stimulated by addition of cytokines. Platelet-derived growth factor and hepatocyte growth factor caused an increase in the migration of cells derived from all three zones, while interleukin-1 selectively stimulated the migration of outer-zone meniscal cells. Epidermal growth factor was much less effective and stimulated the migration of cells in the inner and outer zones by 40% to 50%, while bone morphogenic protein-2 and insulin-like growth factor-1 stimulated the migration of meniscal cells from the middle zone by 40% to 50%. The identification of cytokines that stimulate both the growth and migration of meniscal cells may provide new tools for modulation of meniscal healing.
- Published
- 1999
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