1. Anatomical and molecular characterization of some rhigonematid parasites of millipedes in Nigeria, with new insights into their phylogeny.
- Author
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Fabiyi, O.A., Bello, T.T., Liébanas, G., Clavero-Camacho, I., Cantalapiedra-Navarrete, C., Archidona-Yuste, A., Palomares-Rius, J.E., Hunt, D.J., and Castillo, P.
- Subjects
PHYLOGENY ,MILLIPEDES ,CYTOCHROME oxidase ,RIBOSOMAL DNA - Abstract
Parasitic nematodes of millipedes from Nigeria are molecularly characterized for the first time. During nematode surveys on live giant African millipedes from several localities in Nigeria, 4 species of rhigonematids were identified by application of integrative taxonomical approaches (morpho-anatomy and molecular markers), including Brumptaemilius sp., Gilsonema gabonensis , Obainia pachnephorus , and Rhigonema disparovis. The results of morphometric and molecular analyses of D2-D3 28S, ITS, partial 18S rRNA, and cytochrome oxidase c subunit 1 (COI) gene sequences further characterized the rhigonematid species, and clearly separated them from other related species. Phylogenetic relationships based on 28S and 18S rRNA genes suggest that genera within Ransomnematoidea (Ransomnema , Heth , Carnoya , Brumptaemilius , Cattiena , Insulanema , Gilsonema) and Rhigonematoidea (Rhigonema , Obainia , Xystrognathus , Trachyglossoides , Ichthyocephaloides) clustered rather closer than could be expected in view of their morphological differences. Phylogenetic relationships based on ITS and COI are congruent with those of other ribosomal genes; however, they are not conclusive due to the scarcity of available sequences of these genes for these genera in NCBI. [ABSTRACT FROM AUTHOR] more...
- Published
- 2023
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