1. C to U RNA editing mediated by APOBEC1 requires RNA-binding protein RBM47.
- Author
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Fossat N, Tourle K, Radziewic T, Barratt K, Liebhold D, Studdert JB, Power M, Jones V, Loebel DA, and Tam PP
- Subjects
- APOBEC-1 Deaminase, Animals, Caco-2 Cells, Cell Nucleus metabolism, Cytidine metabolism, Gene Expression, Humans, Mice, Transgenic, Protein Transport, RNA, Messenger genetics, RNA, Messenger metabolism, RNA-Binding Proteins metabolism, Uridine metabolism, Cytidine Deaminase physiology, RNA Editing, RNA-Binding Proteins genetics
- Abstract
Cytidine (C) to Uridine (U) RNA editing is a post-transcriptional modification that is accomplished by the deaminase APOBEC1 and its partnership with the RNA-binding protein A1CF. We identify and characterise here a novel RNA-binding protein, RBM47, that interacts with APOBEC1 and A1CF and is expressed in tissues where C to U RNA editing occurs. RBM47 can substitute for A1CF and is necessary and sufficient for APOBEC1-mediated editing in vitro. Editing is further impaired in Rbm47-deficient mutant mice. These findings suggest that RBM47 and APOBEC1 constitute the basic machinery for C to U RNA editing., (© 2014 The Authors.)
- Published
- 2014
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