1. Intragenomic sequence variation of the ITS-1 region within a single flow-cytometry-counted Cyclospora cayetanensis oocysts.
- Author
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Riner DK, Nichols T, Lucas SY, Mullin AS, Cross JH, and Lindquist HD
- Subjects
- Conserved Sequence, Cyclospora classification, DNA, Protozoan chemistry, Feces parasitology, Flow Cytometry, Humans, Oocysts, Phylogeny, Polymerase Chain Reaction, Sequence Alignment, Cyclospora genetics, Cyclosporiasis parasitology, DNA, Ribosomal Spacer chemistry, Gastroenteritis parasitology
- Abstract
Cyclospora cayetanensis, a protozoan of emerging concern, causes self-limiting gastroenteritis in immune-competent hosts. It has been established that sequence variability exists in the first internal transcribed spacer region (ITS-1) of the ribosomal DNA operon from collections of oocysts obtained from individual or pooled fecal samples. To determine if single oocysts also exhibited ITS-1 sequence variability, DNA was extracted from individually flow-cytometry-counted oocysts. We determined that ITS-1 sequence variability exists at an individual-genome level for C. cayetanensis and approached or exceeded the variability exhibited among oocyst collections. ITS-1 variability, at the genome level, reduces this region's utility for inferring relationships between strains.
- Published
- 2010
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