1. [The protective effect of the amino acids in the action of cytostatic in culture of the lymphoid tissue of young and aged rats].
- Author
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Chalisova NI, Urt'eva SA, Urt'eva TA, Bunina AA, Zhekalov AN, Smirnov AV, and Kontsevaia EA
- Subjects
- Aging pathology, Animals, Apoptosis drug effects, Cell Proliferation drug effects, Dose-Response Relationship, Drug, Male, Rats, Rats, Wistar, Tissue Culture Techniques, Aging drug effects, Amino Acids pharmacology, Cyclophosphamide adverse effects, Cytostatic Agents adverse effects, Protective Agents pharmacology, Spleen drug effects, Spleen pathology
- Abstract
The effect of the cyclophosphane and amino acids with side charge radicales (basic--lysine, arginine, acid--asparagine and glutamic acid) on the cell proliferation and apoptosis development in lymphoide tissue explants in young rats (at age 3 months old) and old rats (at age 24 months old) was investigated in organotypic tissue culture. All amino acids in concentration of 10(-12) M stimulated proliferation in explants' growth zone of young rats and two amino acids (arginine, glutamic acid)--of old rats. Cyclophosphane in concentration of 1 mg/ml inhibited the explant cell proliferation. A delay of the cyclophosphane inhibitory effect was observed under the combined administration into the culture of cyclophosphane with each of the amino acids having a stimulated effect. Thus, the amino acids can possess a protective effect by action of DNA synthesis inhibitors both in young and old rats.
- Published
- 2010