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Your search keyword '"A.M.W.C. van den Broek"' showing total 2 results
Start Over Author "A.M.W.C. van den Broek" Topic cyclooxygenase
2 results on '"A.M.W.C. van den Broek"'

1. Formation of leukotriene B4, 20-hydroxy leukotriene B4 and other arachidonic acid metabolites by macrophages during peritonitis in patients with continuous ambulatory peritoneal dialysis

Author

A.M.C. op de Hoek-Fez, F.J. Zijlstra, Magda Claeys, P.P.H.M. Diderich, J. E. Vincent, and A.M.W.C. van den Broek

Subjects
Male, Physiology, Leukotriene B4, medicine.medical_treatment, Peritonitis, chemistry.chemical_element, Pharmacology, Calcium, Biochemistry, Peritoneal dialysis, chemistry.chemical_compound, Endocrinology, Peritoneal Dialysis, Continuous Ambulatory, medicine, Humans, Chromatography, High Pressure Liquid, Aged, biology, business.industry, Macrophages, Continuous ambulatory peritoneal dialysis, Middle Aged, medicine.disease, Thromboxane B2, chemistry, Fatty Acids, Unsaturated, biology.protein, Kidney Diseases, lipids (amino acids, peptides, and proteins), Arachidonic acid, Cyclooxygenase, business
Abstract

Macrophages, isolated from dialysis fluid of three patients with continuous ambulatory peritoneal dialysis (CAPD) at different times during peritonitis were labelled with 14C-arachidonic acid and stimulated with the calcium ionophore A23187. The main metabolites formed by 5-lipoxygenase activity were leukotriene B4 (LTB4) and 5-hydroxy-6, 9, 11, 14-eicosatetraenoic acid (5-HETE). Smaller amounts of cyclooxygenase metabolites were present and also a major compound with an elution time between 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha) and thromboxane B2 (TxB2). This substance was isolated, analyzed by GC-MS and identified as 20-hydroxy-leukotriene B4 (20-OH-LTB4). This indicates that human peritoneal macrophages obtained from CAPD not only produce leukotrienes and prostaglandins, but also the omega-hydroxylase product of LTB4, which has been demonstrated to be present in polymorphonuclear leucocytes. The activity of this enzyme was not correlated with the severity of the peritonitis.

Published
1987
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2. Comparison of the production of eicosanoids by human and rat peritoneal macrophages and rat Kupffer cells

Author

A. Brouwer, A.M.W.C. van den Broek, J. E. Vincent, J. H. P. Wilson, Freek J. Zijlstra, and R. J. T. Ouwendijk

Subjects
Thromboxane, Leukotriene B4, Kupffer Cells, Eicosatetraenoic acid, Lipoxygenase, Prostaglandin, Arachidonic Acids, Biology, Biochemistry, chemistry.chemical_compound, Endocrinology, Hydroxyeicosatetraenoic Acids, Animals, Ascitic Fluid, Humans, Peritoneal Lavage, Peritoneal Cavity, Chromatography, High Pressure Liquid, Arachidonic Acid, Macrophages, Rats, Thromboxane B2, chemistry, Prostaglandin-Endoperoxide Synthases, biology.protein, Fatty Acids, Unsaturated, Prostaglandins, Arachidonic acid, Female, SRS-A, Cyclooxygenase, Prostaglandin D2
Abstract

Human and rat peritoneal macrophages and rat Kupffer cells were labelled with [1-14C] arachidonic acid and stimulated with the calcium ionophore A23187. The metabolites formed were separated by high pressure liquid chromatography (HPLC). Human peritoneal macrophages formed especially leukotriene B4, 5-hydroxy-6,8,11,14 eicosatetraenoic acid and small amounts of leukotriene C4 and thromboxane B2, 12-hydroxy-5,8,10 heptadecatrienoic acid and 6-keto-prostaglandin F1 alpha, whereas rat peritoneal macrophages mainly produced cyclooxygenase products and in particular thromboxane B2 and 12-hydroxy-5,8,10 heptadecatrienoic acid. Rat Kupffer cells synthesized mainly cyclooxygenase products such as prostaglandin F2 alpha, prostaglandin D2 and prostaglandin E2. These results indicate that the profile of eicosanoids production by macrophages is dependent both on the species and on the tissue from which the macrophage is derived.

Published
1988

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