1. Alpha-lipoic acid modulates GFAP, vimentin, nestin, cyclin D1 and MAP-kinase expression in astroglial cell cultures.
- Author
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Bramanti V, Tomassoni D, Bronzi D, Grasso S, Currò M, Avitabile M, Li Volsi G, Renis M, Ientile R, Amenta F, and Avola R
- Subjects
- Animals, Astrocytes enzymology, Astrocytes metabolism, Blotting, Western, Cells, Cultured, Nestin, Rats, Rats, Wistar, Astrocytes drug effects, Cyclin D1 metabolism, Glial Fibrillary Acidic Protein metabolism, Intermediate Filament Proteins metabolism, Mitogen-Activated Protein Kinases metabolism, Nerve Tissue Proteins metabolism, Thioctic Acid pharmacology, Vimentin metabolism
- Abstract
In the present study, we evaluated the expression of some proliferation and differentiation markers in 15 DIV astrocyte cultures pretreated or not with 0.5 mM glutamate for 24 h and than maintained under chronic or acute treatment with 50 μM R(+)enantiomer or raceme alpha-lipoic acid (ALA). GFAP expression significantly increased after (R+)enantiomer acute-treatment and also in glutamate-pretreated ones. Vimentin expression increased after R(+)enantiomer acute-treatment, but it decreased after raceme acute-treatment. Nestin expression drastically increased after acute raceme-treatment in glutamate-pretreated or not cultures, but significantly decreased after (R+)enantiomer acute and chronic-treatments. Cyclin D1 expression increased in raceme acute-treated cultures pretreated with glutamate. MAP-kinase expression slightly increased after (R+)enantiomer acute treatment in glutamate-pretreated or unpretreated ones. These preliminary findings may better clarify antioxidant and metabolic role played by ALA in proliferating and differentiating astrocyte cultures suggesting an interactive cross-talk between glial and neuronal cells, after brain lesions or damages.
- Published
- 2010
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