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Abstract This work highlights the electrochemical applications of pyridinedicarboxylic acid based Cobalt metal-organic framework (Co-MOF). Cobalt metal-organic framework (Co-MOF), as-synthesized was characterized by the techniques such as ATR-IR, PXRD, SEM, Single crystal XRD, and BET. The Co-MOF electrode was prepared and electrochemical performance was carried out to explore sensing activity of the material for Glucose. The electrochemical sensing experiments were performed in acetate buffer and phosphate buffer separately. An excellent response obtained by the Co-MOF electrode for sensing the Glucose at very low concentrations in acetate buffer. [ABSTRACT FROM AUTHOR]