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Abstract Introduction The aim of the present study was to investigate the impact of ambient temperature on the life span of nickel-titanium (NiTi) files. Methods Cyclic fatigue testing of NiTi instruments was performed in a stainless steel artificial canal. During the experiment, 4 different temperatures (0°C, 20°C, 35°C, and 39°C) were used in a thermostatic bath controlled using electronic and infrared controls. Eighty R25 Reciproc Blue (VDW, Munich, Germany) and 80 R25 Reciproc (VDW) instruments were reciprocated in the artificial canal until fracture occurred, and the time to fracture and the length of the fractured fragment were recorded. The phase transformation temperature for 3 instruments of each type was analyzed by differential scanning calorimetry. One-way analysis of variance and Bonferroni tests were used to statistically analyze the data at a 5% significance level (P <.05). Results Reciproc Blue instruments were significantly more resistant to cyclic fatigue than Reciproc at all the temperatures tested (P <.05). Fatigue resistance was proportionally higher as the environmental temperature decreased (0°C > 20°C > 35°C > 39°C). No significant difference was registered for the length of the fractured fragment of both instruments tested at all temperatures tested (P >.05). Conclusions Blue alloy significantly increases the cyclic fatigue resistance of Reciproc files compared with M-Wire alloy. Temperature significantly affects the life span of NiTi files. When the ambient temperature increases between 0°C and 35°C, the fatigue resistance of the files statistically decreases. Highlights • Environmental temperature may drastically affect the cyclic fatigue resistance of nickel-titanium files. • Reciproc Blue instruments were statistically more resistant to cyclic fatigue than Reciproc M-Wire at all the temperature conditions tested. • The fatigue resistance of both file types tested was proportionally higher as the environmental temperature decreased (0°C > 20°C > 35°C > 39°C), with differences ranging from 35%–421%. • The 2 different instruments analyzed displayed different transformation temperatures. [ABSTRACT FROM AUTHOR]

Introduction The purpose of this study was to evaluate the effect of sodium hypochlorite (NaOCl) and EDTA solutions on the cyclic fatigue resistance of WaveOne (WO) and WaveOne Gold (WOG) nickel-titanium reciprocating files. Methods A hundred WO (25/.08), and 100 WOG (25/.07) were randomly divided into 5 groups: group 1, no immersion; group 2, immersion in 5.25% NaOCl at 37°C ± 1°C for 5 minutes; group 3, immersion in 5.25% NaOCl at 37°C ± 1°C for 10 minutes; group 4, immersion in 17% EDTA at 37°C ± 1°C for 5 minutes; and group 5, immersion in 17 % EDTA at 37°C ± 1°C for 10 minutes. Then, the instruments were reciprocated in an artificial canal until fracture occurred, and the time to fracture was recorded. The data were statistically analyzed using 1-way analysis of variance and the Student t test via SPSS 21.0 software (IBM Corp, Armonk, NY) with the significance level set at P < .05. Results The cyclic fatigue resistance of the WOG was statistically higher than the WO in all the conditions tested ( P < .05). There was no statistically significant difference among the different conditions tested in terms of cyclic fatigue resistance for both WO and WOG files ( P > .05). Among the groups, there was no significant difference in the fracture lengths ( P > .05). Conclusions Within the limitations of the present study, it was determined that NaOCl and EDTA solutions did not have any effect on the cyclic fatigue resistance of WO and WOG files. [ABSTRACT FROM AUTHOR]

Introduction The purpose of this study was to assess the effects of sodium hypochlorite (NaOCl) immersion and sterilization on the cyclic fatigue resistance of heat-treated nickel-titanium (NiTi) rotary instruments. Methods Two hundred ten new 25/.06 Twisted Files (TFs; SybronEndo, Orange, CA) and Hyflex CM (Coltene Whaledent, Cuyahoga Falls, OH) files were divided into 7 groups ( n = 15) for each brand. Group 1 (control group) included new instruments that were not immersed in NaOCl or subjected to autoclave sterilization. Groups 2 and 3 were composed of instruments dynamically immersed for 3 minutes in 5% NaOCl solution 1 and 3 times, respectively. Groups 4 and 5 consisted of instruments only autoclaved 1 and 3 times, respectively. Groups 6 and 7 recruited instruments that received a cycle of both immersion in NaOCl and sterilization 1 and 3 times, respectively. Instruments were subsequently subjected to a fatigue test. The surface morphology of fractured instruments was studied by field-emission scanning electron microscopy and x-ray energy-dispersive spectrometric (EDS) analyses. The means and standard deviations of the number of cycles to failure (NCF) were calculated and statistically analyzed using 2-way analysis of variance ( P < .05). Results Comparison among groups indicated no significant difference of NCF ( P > .05) except for the groups of TFs sterilized 3 times without and with immersion in NaOCl ( P < .05). HyFlex CM files exhibited higher cyclic fatigue resistance than TFs when files were sterilized 3 times, independently from immersion in NaOCl ( P < .05). EDS analysis showed the presence of an oxide-rich layer on the Hyflex CM files’ external surface. No morphologic or chemical differences were found between files of the same brand subjected to different treatments. Conclusions Repeated cycles of sterilization did not influence the cyclic fatigue of NiTi files except for TFs, which showed a significant decrease of flexural resistance after 3 cycles of sterilization. Immersion in NaOCl did not reduce significantly the cyclic fatigue resistance of all heat-treated NiTi files tested. [ABSTRACT FROM AUTHOR]

Introduction The aim of the present study was to analyze how a low environmental temperature can affect the fatigue life of instruments made by different types of heat-treated nickel-titanium alloys. Methods The flexural cyclic fatigue of 40 new specimens for each of the following systems was tested for cyclic fatigue resistance: ProTaper Universal F2 (Dentsply Maillefer, Ballaigues, Switzerland), ProTaper Gold F2 (Dentsply Tulsa Dental Specialties, Tulsa, OK), Twisted Files SM2 (SybronEndo, Orange, CA), Mtwo #25.06 (VDW, Munich, Germany), and Vortex Blue #30.04 and #40.06 (Dentsply Tulsa Dental Specialties). Instruments were tested at 2 different environmental temperatures: 20°C (±2°C) for room temperature (RT) group and −20°C (±2°C) for the cooled environment (CE) group ( n = 20). The number of cycles to failure (NCF) and the length of the fractured fragment (FL) were recorded. The means and standard deviations of NCF and FL were then calculated; NCF data were statistically analyzed using a paired t test between groups RT and CE for each instrument tested ( P < .05), whereas FL data were analyzed using analysis of variance ( P < .05). Results The mean NCF values measured were significantly higher for the CE groups than the RT groups in all the systems tested ( P < .05). The increase in cyclic fatigue resistance varied from 274%–854%. No differences in FL were registered among the different groups ( P < .05). Conclusions A low environmental temperature determines a drastic increase in the flexural fatigue resistance of NiTi endodontic instruments manufactured with traditional alloy and different heat treatments. [ABSTRACT FROM AUTHOR]

Introduction The aim of the present study was to evaluate the influence of Blue thermal treatment on the bending resistance and cyclic fatigue of conventional M-Wire Reciproc files (VDW, Munich, Germany). The roughness pattern and the microhardness of the files were also assessed. Methods Flexibility of standard Reciproc R25 files and the corresponding Blue prototypes was determined by 45° bending tests according to the ISO 3630-1 specification. Instruments were also subjected to cyclic fatigue resistance, measuring the time to fracture in an artificial stainless steel canal with a 60° angle and a 5-mm radius of curvature. The fracture surface of all fragments was examined with a scanning electron microscope. The roughness of the working parts was quantified by using a profilometer, and the microhardness test was performed using the Vickers hardness tester. Results were statistically analyzed using the Student t test with a level of significance set at P < .05. Results Reciproc Blue instruments presented a significantly longer cyclic fatigue life and significantly lower bending resistance than the original Reciproc instrument ( P < .05). Regarding the roughness pattern, there was no significant difference between Reciproc Blue and the original Reciproc instruments ( P > .05), whereas Reciproc Blue revealed significantly lower microhardness than the original Reciproc instrument ( P < .05). Conclusions Reciproc Blue nickel-titanium showed improved all-around performance when compared with conventional M-Wire superelastic nickel-titanium, demonstrating improved flexibility and fatigue resistance, and reduced microhardness while maintaining similar characteristics of the surface. [ABSTRACT FROM AUTHOR]

Introduction: The purpose of this study was to evaluate the torsional and cyclic fatigue resistance of the new Hyflex EDM OneFile (Coltene/Whaledent AG, Altstatten, Switzerland) manufactured by electrical discharge machining and compare the findings with the ones of Reciproc R25 (VDW, Munich, Germany) and WaveOne Primary (Dentsply Maillefer, Ballaigues, Switzerland). Methods: One hundred-twenty new Hyflex EDM One- File (#25/0.08), Reciproc R25, and WaveOne Primary files were used. Torque and angle of rotation at failure of new instruments (n = 20) were measured according to ISO 3630-1 for each brand. Cyclic fatigue resistance was tested measuring the number of cycles to failure in an artificial stainless steel canal with a 60° angle and a 3-mm radius of curvature. Data were analyzed using the analysis of variance test and the Student- Newman-Keuls test for multiple comparisons. The fracture surface of each fragment was examined with a scanning electron microscope. Results: The cyclic fatigue of Hyflex EDM was significantly higher than the one of Reciproc R25 and WaveOne Primary (P < .05 and P < .001, respectively). Hyflex EDM showed a lower maximum torque load (P < .05) but a significantly higher angular rotation (P < .0001) to fracture than Reciproc R25 and WaveOne Primary. No significant difference was found comparing the maximum torque load, angular rotation, and cyclic fatigue of Reciproc R25 and WaveOne Primary (P > .05). Conclusions: The new Hyflex EDM instruments (controlled memory wire) have higher cyclic fatigue resistance and angle of rotation to fracture but lower torque to failure than Reciproc R25 and WaveOne Primary files (M-wire for both files). [ABSTRACT FROM AUTHOR]

Introduction The aim of this study was to evaluate the cyclic fatigue resistance of ProTaper Universal (PTU; Dentsply Maillefer, Baillagues, Switzerland) and ProTaper Gold (PTG, Dentsply Maillefer) nickel-titanium rotary files at room and intracanal temperatures. Methods A total of 120 files were used to test the cyclic fatigue resistance of PTU (S1 and F2) and PTG (S1 and F2) in an artificial canal at room temperature (20°C) and at intracanal temperature (35°C). The number of cycles to failure and the fracture length of the fragment were recorded, and data were subjected to statistical analysis. Results PTG registered no differences in fatigue life between the 2 temperatures tested ( P > .05), whereas PTU showed a statistically significant reduction in fatigue life at intracanal temperature compared with room temperature ( P < .05). PTG instruments exhibited a statistically higher resistance to cyclic fatigue than PTU instruments both at room and intracanal temperatures ( P < .05). There were no statistically significant differences among the fracture length of separated instruments ( P > .05). Conclusions Intracanal temperature influenced the cyclic fatigue resistance of instruments produced with traditional nickel-titanium, whereas it did not influence the fatigue life of instruments produced with gold heat treatment. Gold heating treatment enhances the resistance to cyclic fatigue of ProTaper instruments. [ABSTRACT FROM AUTHOR]

Abstract: Introduction: The aim of this study was to evaluate the resistance to flexural fatigue of Reciproc R25 (VDW, Munich, Germany), WaveOne Primary (VDW, Munich, Germany), Mtwo (Sweden & Martina, Padova, Italy), and Twisted File (TF; SybronEndo, Orange, CA) instruments used in continuous rotation or in 2 different reciprocating motions. Methods: A total of 180 nickel-titanium files from 4 brands marketed, 2 for use in reciprocating motion (ie, Reciproc R25 and WaveOne Primary) and 2 for use in continuous rotation (ie, Mtwo and TF both taper .06/0.25 tip diameter), were tested. Forty-five instruments for each brand were divided into 3 groups (n = 15) on the basis of the motion tested: continuous rotation (group 1: 300 rpm) and reciprocal motion (group 2: “RECIPROC ALL” mode and group 3: “WAVEONE ALL” mode). The resistance to cyclic fatigue was determined by counting the number of cycles to failure in a 60° curve with a 5-mm radius. Data were analyzed through 2-way analyses of variance. Results: The cyclic fatigue resistance of the 2 reciprocating motion instruments (ie, “RECIPROC ALL” and “WAVEONE ALL”) was significantly higher than the continuous rotation in each brand (P < .001). No significant difference was observed in cyclic fatigue between the 2 different reciprocal motions tested in each brand (P > .05). When considering the appropriate clinical motion for each brand, no significant difference in cyclic fatigue was found between TF, Mtwo, and Reciproc R25 instruments, whereas the cyclic fatigue resistance of WaveOne files was less than the other 3 brands (P < .05). Conclusions: Reciprocal motion showed a significantly higher cyclic fatigue resistance in all brands compared with continuous rotation. No differences were found between the 2 reciprocating motions. [Copyright &y& Elsevier]

Abstract: Introduction: The purpose of this study was to evaluate the effect of autoclave sterilization on cyclic fatigue resistance of rotary endodontic instruments made of traditional and new nickel-titanium (NiTi) alloys. Methods: Four NiTi rotary endodontic instruments of the same size (tip diameter 0.40 mm and constant .04 taper) were selected: K3, Mtwo, Vortex, and K3 XF prototypes. Each group was then divided into 2 subgroups, unsterilized instruments and sterilized instruments. The sterilized instruments were subjected to 10 cycles of autoclave sterilization. Twelve files from each different subgroup were tested for cyclic fatigue resistance. Means and standard deviations of number of cycles to failure (NCF) and fragment length of the fractured tip were calculated for each group, and data were statistically analyzed (P < .05). Results: Comparing the results between unsterilized and sterilized instruments for each type of file, differences were statistically significant (P < .05) only between sterilized and unsterilized K3XF files (762 versus 651 NCF). The other instruments did not show significant differences (P > .05) in the mean NCF as a result of sterilization cycles (K3, 424 versus 439 NCF; Mtwo, 409 versus 419 NCF; Vortex, 454 versus 480 NCF). Comparing the results among the different groups, K3 XF (either sterilized or not) showed a mean NCF significantly higher than all other files (P < .05). Conclusions: Repeated cycles of autoclave sterilization do not seem to influence the mechanical properties of NiTi endodontic instruments except for the K3 XF prototypes of rotary instruments that demonstrated a significant increase of cyclic fatigue resistance. [Copyright &y& Elsevier]

Abstract: Introduction: The purpose of this study was to assess the resistance to cyclic fatigue of three nickel-titanium (NiTi) files after the immersion in sodium hypochlorite (NaOCl) solution in conditions similar to those used in clinical practice. Methods: A total of 150 new Twisted Files (SybronEndo, Orange, CA), Revo S SU files (Micro Mega, Besancon, France), and Mtwo files (Sweden and Martina, Padova, Italy), size 25.06, were tested. Fifty files of the same brand were randomly assigned to five groups (n = 10) and submitted to the following immersion protocol in 5% NaOCl at 37°C for 16 mm: no immersion (control), 5 minutes statically, 1 minute statically, 5 minutes dynamically (300 rpm/min), and 1 minute dynamically. Resistance to cyclic fatigue was determined by counting the numbers of cycles to failure in a 60° curve with a 5-mm radius. Data were analyzed by two-way analyses of variance. Results: Resistance to cyclic fatigue of the same NiTi file was not significantly affected by immersion in NaOCl (P > .05). The Twisted File showed a higher resistance in all groups than Revo S SU (P < .001). The comparison between the same groups of Twisted Files and Mtwo files or between Mtwo and Revo S files did not show significant differences (P > .05) except for two cases: group 2 of the Twisted Files and Mtwo files and group 5 of the Mtwo and Revo S SU files (P < .05). Conclusions: Static or dynamic immersion in NaOCl for 1 minute or 5 minutes did not reduce the cyclic fatigue resistance of NiTi significantly. However, the type of instrument influences cyclic fatigue resistance. In our study, Twisted Files were more resistant followed by Mtwo and Revo S SU files. [Copyright &y& Elsevier]

Abstract: Introduction: Fractured rotary nickel-titanium (NiTi) instruments have been classified into those that fail as a result of cyclic flexural fatigue or torsional failure or a combination of both. Clinically, NiTi rotary instruments are subjected to both torsional load and cyclic fatigue, and ongoing research aims to clarify the relative contributions of both factors to instrument separation. Methods: To date, there is no specification or international standard to test cyclic fatigue resistance of endodontic rotary instruments. As a consequence, several devices and methods have been used to investigate in vitro cyclic fatigue fracture resistance of NiTi rotary endodontic instruments. In nearly all studies reported in the endodontic literature, the rotating instrument was either confined in a glass or metal tube, in a grooved block-and-rod assembly, or in a sloped metal block. Results: There has been no mention of the “fit” of the instrument in the tube or groove. As the instrument is likely to be fitting loosely, the description of the radius of curvature in those studies is likely to be overstated (ie, the file was actually bent less severely than reported, adding a variability in the amount of flexural stress). Conclusions: This review analyzed several devices that have been used in endodontic literature for cyclic fatigue testing and found that differences in the methodology affected the fatigue behavior of rotary instruments and, consequently, the outcome of these studies. An international standard for cyclic fatigue testing of NiTi rotary instruments is required to ensure uniformity of methodology and comparable results. [Copyright &y& Elsevier]

Introduction The purpose of this study was to evaluate the cyclic fatigue resistance of 3 different nickel-titanium reciprocating instruments. Methods A total of 45 nickel-titanium instruments were tested and divided into 3 experimental groups ( n = 15): group 1, WaveOne Primary instruments; group 2, Reciproc R25 instruments; and group 3, Twisted File (TF) Adaptive M-L1 instruments. The instruments were then subjected to cyclic fatigue test on a static model consisting of a metal block with a simulated canal with 60° angle of curvature and a 5-mm radius of curvature. WaveOne Primary, Reciproc R25, and TF Adaptive instruments were activated by using their proprietary movements, WaveOne ALL, Reciproc ALL, and TF Adaptive, respectively. All instruments were activated until fracture occurred, and the time to fracture was recorded visually for each file with a 1/100-second chronometer. Mean number of cycles to failure and standard deviations were calculated for each group, and data were statistically analyzed ( P < .05). Instruments were also observed through scanning electron microscopy to evaluate type of fracture. Results Cyclic fatigue resistance of Reciproc R25 and TF Adaptive M-L1 was significantly higher than that of WaveOne Primary ( P = .009 and P = .002, respectively). The results showed no statistically significant difference between TF Adaptive M-L1 and Reciproc R25 ( P = .686). Analysis of the fractured portion under scanning electron microscopy indicated that all instruments showed morphologic characteristics of ductile fracture that were due to accumulation of metal fatigue. Conclusions No statistically significant differences were found between the instruments tested except for WaveOne Primary, which showed the lowest resistance to cyclic fatigue. [ABSTRACT FROM AUTHOR]

Introduction The aim of the present study was to evaluate the difference in cyclic fatigue resistance between Vortex Blue (Dentsply Tulsa Dental, Tulsa, OK) and Profile Vortex nickel-titanium (Dentsply Tulsa Dental) rotary instruments. Methods Two groups of nickel-titanium endodontic instruments, ProFile Vortex and Vortex Blue, consisting of identical instruments in tip size and taper (15/.04, 20/.06, 25/.04, 25/.06, 30/.06, 35/.06, and 40/.04) were tested. Ten instruments from each system and size were tested for cyclic fatigue resistance, resulting in a total of 140 new instruments. All instruments were rotated in a simulated root canal with a 60° angle of curvature and a 5-mm radius of curvature of a specific cyclic fatigue testing device until fracture occurred. The number of cycles to failure and the length of the fractured tip were recorded for each instrument in each group. The mean values and standard deviation were calculated, and data were subjected to 1-way analysis of variance and a Bonferroni t test. Significance was set at the 95% confidence level. Results When comparing the same size of the 2 different instruments, a statistically significant difference (P < .05) was noted between all sizes of Vortex Blue and Profile Vortex instruments except for tip size 15 and .04 taper (P = 1.000). No statistically significant difference (P > .05) was noted among all groups tested in terms of fragment length. Conclusions Vortex Blue showed a significant increase in cyclic fatigue resistance when compared with the same sizes of ProFile Vortex. [ABSTRACT FROM AUTHOR]

Abstract: Introduction: The goal of the present study was to test the fatigue resistance of nickel-titanium rotary files in a double curvature (S-shaped) artificial root canal and to compare those results with single curvature artificial root canals. Methods: Two nickel-titanium endodontic instruments consisting of identical instrument sizes (constant .06 taper and 0.25 tip diameter) were tested, ProFile instruments and Vortex instruments. Both instruments were tested for fatigue inside an artificial canal with a double curvature and inside a curved artificial canal with a single curvature. Ten instruments for each group were tested to fracture in continuous rotary motion at 300 rpm. Number of cycles to failure (NCF) was calculated to the nearest whole number, and the length of the fractured fragment was measured in millimeters. Data were statistically analyzed with a level of significance set at 95% confidence level. Results: The NCF value was always statistically lower in the double curved artificial canal when compared with the single curve (P < .05) in both the apical and coronal curvatures. Statistically significant differences (P < .05) were noted between instruments of the same size of different brand only in the single curve; ProFile registered a mean of 633.5 ± 75.1 NCF, whereas Vortex registered a mean of 548 ± 48.9 NCF. Conclusions: Regardless of the differences between the instruments used in the present study, the results suggest that the more complex is the root canal, the more adverse are the effects on the cyclic fatigue resistance of the instruments. [Copyright &y& Elsevier]

Abstract: The aim of the present study was to investigate whether cyclic fatigue resistance is increased for nickel-titanium instruments manufactured by using new processes. This was evaluated by comparing instruments produced by using the twisted method (TF; SybronEndo, Orange, CA) and those using the M-wire alloy (GTX; Dentsply Tulsa-Dental Specialties, Tulsa, OK) with instruments produced by a traditional NiTi grinding process (K3, SybronEndo). Tests were performed with a specific cyclic fatigue device that evaluated cycles to failure of rotary instruments inside curved artificial canals. Results indicated that size 06-25 TF instruments showed a significant increase (p < 0.05) in the mean number of cycles to failure when compared with size 06-25 K3 files. Size 06-20 K3 instruments showed no significant increase (p > 0.05) in the mean number of cycles to failure when compared with size 06-20 GT series X instruments. The new manufacturing process produced nickel-titanium rotary files (TF) significantly more resistant to fatigue than instruments produced with the traditional NiTi grinding process. Instruments produced with M-wire (GTX) were not found to be more resistant to fatigue than instruments produced with the traditional NiTi grinding process. [Copyright &y& Elsevier]