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7 results on '"Vydelingum N"'

1. The role of calcium in insulin action. I. Purification and properties of enzymes regulating lipolysis in human adipose tissue: effects of cyclic-AMP and calcium ions.

Author

Kissebah AH, Vydelingum N, Tulloch BR, Hope-Gill H, and Fraser TR

Subjects
Adipose Tissue cytology, Adipose Tissue drug effects, Adipose Tissue enzymology, Binding Sites, Chromatography, DEAE-Cellulose, Cyclic AMP metabolism, Cyclic GMP pharmacology, Cytosol enzymology, Enzyme Activation, Humans, Hydrogen-Ion Concentration, In Vitro Techniques, Inosine Nucleotides, Kinetics, Lipase antagonists & inhibitors, Lipase isolation & purification, Microsomes enzymology, Mitochondria enzymology, Nucleotides, Cyclic pharmacology, Protein Binding, Protein Kinases metabolism, Subcellular Fractions enzymology, Time Factors, Triglycerides, Adipose Tissue metabolism, Calcium pharmacology, Cyclic AMP pharmacology, Insulin metabolism, Lipase metabolism, Lipid Mobilization drug effects
Published
1974
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2. Cyclic adenosine monophosphate in human adipose tissue.

Author

Tulloch BR, Vydelingum N, and Galton DJ

Subjects
Acetanilides pharmacology, Adenylyl Cyclases metabolism, Adrenal Glands metabolism, Adrenergic beta-Antagonists pharmacology, Amino Alcohols pharmacology, Animals, Blood Glucose, Cattle, Fluorides pharmacology, Glucose Tolerance Test, Humans, Isoproterenol pharmacology, Magnesium pharmacology, Obesity metabolism, Phosphoric Diester Hydrolases metabolism, Propranolol pharmacology, Protein Binding, Theophylline pharmacology, Adipose Tissue metabolism, Cyclic AMP metabolism
Published
1972

3. The effect of procaine hydrochloride and insulin on the cyclic AMP mediated responses in rat adipose tissue.

Author

Fraser TR, Hope-Gill H, Kissebah A, Tulloch BR, and Vydelingum N

Subjects
Adipose Tissue metabolism, Animals, Biological Transport, Active drug effects, Calcium physiology, Fatty Acids biosynthesis, Fructose metabolism, Glucose metabolism, Glycogen biosynthesis, In Vitro Techniques, Lipid Metabolism, Rats, Adipose Tissue drug effects, Cyclic AMP physiology, Insulin pharmacology, Procaine pharmacology
Published
1973

4. Simulation and enhancement of the adipose tissue insulin response by procaine hydrochloride: evidence for a role of calcium in insulin action

Author

T. R. Fraser, B. Tulloch, A. H. Kissebah, Vydelingum N, and Hope-Gill H

Subjects
medicine.medical_specialty, Time Factors, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Clinical Biochemistry, Pentoses, chemistry.chemical_element, Adipose tissue, Procaine Hydrochloride, Biological Transport, Active, White adipose tissue, Fructose, Calcium, Pharmacology, Biochemistry, Endocrinology, Theophylline, Internal medicine, Insulin response, medicine, Cyclic AMP, Animals, Insulin, Pyruvates, Biochemistry (medical), Drug Synergism, General Medicine, Lipids, Rats, Glucose, chemistry, Adipose Tissue, Glycogen, Procaine
Published
1974

5. The role of calcium in insulin action. I. Purification and properties of enzymes regulating lipolysis in human adipose tissue: effects of cyclic-AMP and calcium ions

Author

A. H. Kissebah, T. R. Fraser, B. Tulloch, Hope-Gill H, and Vydelingum N

Subjects
medicine.medical_specialty, Time Factors, Endocrinology, Diabetes and Metabolism, Clinical Biochemistry, Phosphatase, Adipose tissue, chemistry.chemical_element, Hormone-sensitive lipase, Calcium, In Vitro Techniques, Biochemistry, Chromatography, DEAE-Cellulose, Endocrinology, Cytosol, Internal medicine, Microsomes, medicine, Cyclic AMP, Lipolysis, Humans, Insulin, Lipase, Protein kinase A, Cyclic GMP, Inosine Nucleotides, Triglycerides, Lipoprotein lipase, Binding Sites, biology, Biochemistry (medical), Lipid Mobilization, General Medicine, Hydrogen-Ion Concentration, Mitochondria, Enzyme Activation, Kinetics, chemistry, Adipose Tissue, biology.protein, Nucleotides, Cyclic, Protein Kinases, Protein Binding, Subcellular Fractions
Abstract

The reaction steps involved in the activation and inactivation of the hormone sensitive lipase have been studied in human adipose tissue. Homogenates prepared from adipose tissue shreds or isolated fat cells contained a cyclic-AMP dependant protein kinase. This enzyme was partially purified from the 100,000 g supernatant using DEAE Cellulose Column Chromatography. The activity of the purified preparation was stimulated 3-4 fold with cyclic-AMP and inhibited with progressive increases in free calcium concentration in the reaction mixture. Calcium seems to inhibit the binding of cyclic-AMP to the protein kinase regulatory units and thus abolishes cyclic-AMP stimulation. In the presence of cyclic-AMP and ATP the protein kinase phosphorylates a lipase preparation partially purified from the same tissue with synchronous increase in the lipolytic activity. Inactivation of the lipase is preceeded by its dephosphorylation by a phosphatase(s) which is present in 105,000 g supernatant. Increasing free calcium concentration between 10-8 and 10-3M produced progressive inhibition of the lipase activity. This inhibition can be attributed to a dual mechanism. First, calcium ions inhibited the protein kinase activation of the lipase. Second, calcium ions stimulated the adipose tissue phosphatase(s) and this increased the rate of inactivation of the hormone sensitive lipase. A role for calcium ions as well as cyclic-AMP in the control of lipolysis is postulated.

Published
1974

6. The role of calcium in insulin action. II. Effects of insulin and procaine hydrochloride on lipolysis

Author

Vydelingum N, B. Tulloch, P. Clarke, A. H. Kissebah, T. R. Fraser, and Hope-Gill H

Subjects
Glycerol, Male, medicine.medical_specialty, Epinephrine, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Clinical Biochemistry, chemistry.chemical_element, Adipose tissue, Procaine Hydrochloride, Calcium, In Vitro Techniques, Tritium, Biochemistry, Histones, Procaine, Enzyme activator, Radioligand Assay, Endocrinology, Internal medicine, medicine, Cyclic AMP, Lipolysis, Animals, Insulin, Protein Kinase Inhibitors, Dose-Response Relationship, Drug, Chemistry, Biochemistry (medical), Lipid Mobilization, General Medicine, Phosphoric Monoester Hydrolases, Rats, Enzyme Activation, Spectrometry, Fluorescence, Adipose Tissue, Phosphorus Radioisotopes, Protein Kinases, medicine.drug, Protein Binding
Published
1974

7. Regulation of Glycogenolysis Through Changes in Intracellular Calcium

Author

A. H. Kissebah, P. Clarke, Hope-Gill H, Vydelingum N, B. Tulloch, and Fraser R

Subjects
medicine.medical_specialty, Glycogenolysis, Endocrinology, Diabetes and Metabolism, Clinical Biochemistry, Carboxylic Acids, In Vitro Techniques, Biochemistry, Calcium in biology, Endocrinology, Lanthanum, Internal medicine, Cyclic AMP, medicine, Animals, Chemistry, Biochemistry (medical), General Medicine, Glucagon, Anti-Bacterial Agents, Rats, Calcium ATPase, Glucose, Liver, Calcium, Glycogen
Published
1974
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