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A fluorescent probe that responds at distinct wavelengths upon exposure to cyanide, hypochlorite, and bisulfite was synthesized. As a result, an easy to apply analytical methodology was developed for the detection of these ions. The feasibility of this method was evaluated by theoretical calculations. The probe exhibited excellent solubility in the test solution (H 2 O: DMF = 99: 1, v: v) with low detection limits for cyanide, hypochlorite and bisulfite (4.5 × 10 -8  M, 4.9 × 10 -7  M and 4.3 × 10 -8  M respectively) showing distinct emission wavelengths for each ion without interference in practical application. Furthermore, the probe had low toxicity and was applied for the imaging experiments of cyanide, hypochlorite and bisulfite in living HeLa and MDCK cells., Competing Interests: Declaration of competing interest Conflicts of interest: “There are no conflicts to declare”., (Copyright © 2019. Published by Elsevier Inc.)

A dual-channel fluorescent probe PHTP with excellent water solubility and detection limit was used. The detection selectivity of the probe with CN− or HSO 3 − was determined by the pH value of the solution. Probe reacted with HSO 3 − at the pH of solution was 7.4 and emit a fluorescence signal at 450 nm, but probe reacted with CN− at pH of 9.3 and an intense fluorescence emission with a peak at 507 nm was acquired, and mutual interference observed during the response process was scarce. In addition, other competitive biologically relevant species, including F─, Cl─, Br─, I─, NO 3 ─, SO 4 2─, SCN─, H 2 PO 4 ─, CO 3 2─, C 2 O 4 2─, AcO─, N 3 ─, NO 2 ─, HCO 3 ─, S 2 O 3 2─, HS─, Cys, GSH, and Hcy, were also rare interfere. The reaction mechanism was investigated by direct HR-MS, 1H NMR and extensive theoretical calculation. Furthermore, the cytotoxicity of PHTP by the standard MTT assay has been examined, PHTP had good biocompatibility and low cytotoxicity, and the probe was applied in the detection of living cells. • The sensor with low limits of detection (for cyanide, LOD = 9.8 × 10 −8 M; for bisulfite, LOD = 3.0 × 10 −8 M). • The reaction activity for CN−/HSO 3 − could be regulated by pH for avoiding the mutual interference and competition. • The reaction mechanism was investigated by HR-MS, 1H NMR, and theoretical calculation. • The sensor exhibited excellent water solubility and applied in the detection of living cells. [ABSTRACT FROM AUTHOR]