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Your search keyword '"Mita, Luigi"' showing total 3 results
Start Over Author "Mita, Luigi" Topic curcumin
3 results on '"Mita, Luigi"'

1. Nano-precipitated curcumin loaded particles: effect of carrier size and drug complexation with (2-hydroxypropyl)-β-cyclodextrin on their biological performances.

Author

Serri C, Argirò M, Piras L, Mita DG, Saija A, Mita L, Forte M, Giarra S, Biondi M, Crispi S, and Mayol L

Subjects
2-Hydroxypropyl-beta-cyclodextrin, Cell Line, Tumor, Cell Survival drug effects, Cells, Cultured, Chemical Precipitation, Curcumin administration & dosage, Curcumin pharmacokinetics, Drug Liberation, Drug Stability, Humans, Lactic Acid chemistry, Nanoparticles metabolism, Particle Size, Poloxamer chemistry, Polyglycolic Acid chemistry, Polylactic Acid-Polyglycolic Acid Copolymer, Curcumin chemistry, Curcumin pharmacology, Drug Carriers chemistry, Nanoparticles chemistry, beta-Cyclodextrins chemistry
Abstract

In this work, curcumin (CURC)-encapsulating nanoparticles (NPs), made up of an amphiphilic blend of poloxamers and PLGA (PPC NPs) at different polymer concentrations, were prepared by nanoprecipitation. CURC was preliminarily complexed with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) to improve its loading efficiency. The formation of host-guest complexes of CURC with HPβCD (CD-CURC) was confirmed by means of 1 HNMR studies and differential scanning calorimetry (DSC). Nanoprecipitation allowed to obtain NPs with a small size (90-120nm depending on the polymer concentration), a narrow size distribution and stable in water for 30days at 4°C and in RPMI-1640 cell culture medium up to 72h at 37°C. The in vitro release of CD-CURC, sustained up to 5days, was governed mainly by a diffusive mechanism. It was also found that the produced NPs were efficiently internalized by mesothelioma cells (MSTO-211H) in the cytoplasmic space, at an extent strongly dependent on NP size and polydispesity index, therefore pointing at the importance of NP preparation method in improving their uptake., (Copyright © 2017 Elsevier B.V. All rights reserved.)

Published
2017
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2. Curcumin loaded PLGA-poloxamer blend nanoparticles induce cell cycle arrest in mesothelioma cells.

Author

Mayol L, Serri C, Menale C, Crispi S, Piccolo MT, Mita L, Giarra S, Forte M, Saija A, Biondi M, and Mita DG

Subjects
Antineoplastic Agents, Phytogenic chemistry, Antineoplastic Agents, Phytogenic metabolism, Cell Line, Tumor, Cell Proliferation drug effects, Chemistry, Pharmaceutical, Curcumin chemistry, Curcumin metabolism, Drug Stability, Drug Tolerance, Humans, Kinetics, Mesothelioma pathology, Nanomedicine, Particle Size, Polylactic Acid-Polyglycolic Acid Copolymer, Protein Binding, Solubility, Surface Properties, Technology, Pharmaceutical methods, Antineoplastic Agents, Phytogenic pharmacology, Cell Cycle Checkpoints drug effects, Curcumin pharmacology, Drug Carriers, Lactic Acid chemistry, Mesothelioma drug therapy, Nanoparticles, Poloxamer chemistry, Polyglycolic Acid chemistry
Abstract

The pharmacological potential of curcumin (CURC) is severely restricted because of its low water solubility/absorption, short half-life and poor bioavailability. To overcome these issues, CURC-loaded nanoparticles (NPs) were produced by a double emulsion technique. In particular, NPs were made up of an amphiphilic blend of poloxamers and PLGA to confer stealth properties to the NPs to take advantage of the enhanced permeability and retention (EPR) effect. Different surface properties of NPs made up of bare PLGA and PLGA/poloxamer blend were confirmed by the different interactions of these NPs with serum proteins and also by their ability to be internalized by mesothelioma cell line. The uptake of PLGA/poloxamer NPs induces a persistent block in G0/G1 phase of the cell cycle up to 72 h, thus overcoming the drug tolerance phenomenon, normally evidenced with free CURC., (Copyright © 2015 Elsevier B.V. All rights reserved.)

Published
2015
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3. Nano-precipitated curcumin loaded particles: effect of carrier size and drug complexation with (2-hydroxypropyl)-β-cyclodextrin on their biological performances

Author

Mario Argirò, Simona Giarra, Linda Piras, Antonina Saija, Luigi Mita, Marco Biondi, Carla Serri, Damiano Gustavo Mita, Laura Mayol, Maurizio Forte, Stefania Crispi, Serri, Carla, Argirò, Mario, Piras, Linda, Mita, Damiano Gustavo, Saija, Antonina, Mita, Luigi, Forte, Maurizio, Giarra, Simona, Biondi, Marco, Crispi, Stefania, and Mayol, Laura

Subjects
Mesothelioma, Curcumin, Stereochemistry, Cell Survival, Pharmaceutical Science, Nanoparticle, 02 engineering and technology, Poloxamer, 010402 general chemistry, 01 natural sciences, Poloxamers, chemistry.chemical_compound, Differential scanning calorimetry, Drug Stability, Polylactic Acid-Polyglycolic Acid Copolymer, Cell Line, Tumor, Amphiphile, Cyclodextrin, Chemical Precipitation, Humans, Lactic Acid, Particle Size, Cells, Cultured, chemistry.chemical_classification, Drug Carriers, beta-Cyclodextrins, PLGA, Polymer, 021001 nanoscience & nanotechnology, 0104 chemical sciences, 2-Hydroxypropyl-beta-cyclodextrin, Curcumin, Cyclodextrin, Mesothelioma, Nanoparticles, PLGA, Poloxamers, Drug Liberation, chemistry, Nanoparticles, 0210 nano-technology, Polyglycolic Acid, Nuclear chemistry
Abstract

In this work, curcumin (CURC)-encapsulating nanoparticles (NPs), made up of an amphiphilic blend of poloxamers and PLGA (PPC NPs) at different polymer concentrations, were prepared by nanoprecipitation. CURC was preliminarily complexed with (2-hydroxypropyl)-β-cyclodextrin (HPβCD) to improve its loading efficiency. The formation of host-guest complexes of CURC with HPβCD (CD-CURC) was confirmed by means of 1HNMR studies and differential scanning calorimetry (DSC). Nanoprecipitation allowed to obtain NPs with a small size (90–120 nm depending on the polymer concentration), a narrow size distribution and stable in water for 30 days at 4 °C and in RPMI-1640 cell culture medium up to 72 h at 37 °C. The in vitro release of CD-CURC, sustained up to 5 days, was governed mainly by a diffusive mechanism. It was also found that the produced NPs were efficiently internalized by mesothelioma cells (MSTO-211H) in the cytoplasmic space, at an extent strongly dependent on NP size and polydispesity index, therefore pointing at the importance of NP preparation method in improving their uptake.

Published
2016

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