1. Incidence and molecular diversity of poleroviruses infecting cucurbit crops and weed plants in Thailand.
- Author
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Cheewachaiwit S, Warin N, Phuangrat B, Rukpratanporn S, Gajanandana O, Balatero CH, and Chatchawankanphanich O
- Subjects
- Crops, Agricultural virology, Genome, Viral, Luteoviridae classification, Luteoviridae isolation & purification, Open Reading Frames, Phylogeny, Sequence Analysis, DNA, Thailand, Cucurbita virology, Luteoviridae genetics, Plant Diseases virology, Plant Weeds virology, RNA-Dependent RNA Polymerase genetics
- Abstract
Overall, 244 samples of cucurbit crops with yellowing symptoms and selected weed species, from 15 provinces in Thailand, were screened by RT-PCR using primers Polero-CP-F and Polero-CP-R. A total of 160 samples (~66%) were infected by poleroviruses. Analysis of a 1.4 kb region covering the 3' RNA-dependent RNA polymerase (RdRp) gene, the intergenic non-coding region (iNCR), and the coat protein (CP), showed that four poleroviruses, namely, cucurbit aphid-borne yellows virus (CABYV), luffa aphid-borne yellows virus (LABYV), melon aphid-borne yellows virus (MABYV) and suakwa aphid-borne yellows virus (SABYV) were associated with the yellowing symptoms in cucurbit crops. Further analyses indicated presence of putative recombinant viruses referred to as CABYV-R and SABYV-R. CABYV-R was derived from the recombination between MABYV and the common strain of CABYV (CABYV-C). SABYV-R was derived from the recombination of MABYV and SABYV.
- Published
- 2017
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