1. Phylogenetic analysis of the L and HN gene of ophidian paramyxoviruses. Brief report.
- Author
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Kindermann J, Kübber-Heiss A, Kerschbaumer P, and Nowotny N
- Subjects
- Amino Acid Sequence, Animals, Evolution, Molecular, HN Protein isolation & purification, Mammals virology, Molecular Sequence Data, Parainfluenza Virus 1, Human genetics, Parainfluenza Virus 2, Human genetics, Paramyxoviridae classification, Paramyxoviridae isolation & purification, Paramyxoviridae Infections virology, Phylogeny, Polymerase Chain Reaction, RNA, Viral genetics, Respirovirus genetics, Rubulavirus classification, Rubulavirus genetics, Rubulavirus isolation & purification, Sequence Alignment, Sequence Homology, Amino Acid, Species Specificity, Viral Proteins isolation & purification, Crotalus virology, Genes, Viral, HN Protein genetics, Paramyxoviridae genetics, Paramyxoviridae Infections veterinary, Viperidae virology, Viral Proteins genetics, Viral Structural Proteins genetics
- Abstract
Two reptilian paramyxoviruses, isolated from a neotropical rattlesnake (neotropical virus, NTV, ATCC VR-1408) and a bush viper (bush viper virus, BVV, ATCC VR- 1409), respectively, were analysed to determine their taxonomic position among other reptilian paramyxoviruses investigated previously by Ahne et al.. A 679 bp long region of the hemagglutinin-neuraminidase (HN) gene and a 627 bp long region of the large (L) gene were reverse transcribed, amplified by polymerase chain reaction (PCR), and sequenced. The deduced amino acid sequences were compared to mammalian paramyxoviruses belonging to the genera Respirovirus and Rubulavirus. The deduced amino acid sequences revealed 58.9 to 62% homology for the partial L protein and 41% to 47.1% homology for the partial HN protein. For phylogenetic analyses, a 518 bp L gene and a 352 bp HN gene fragment were used, both generating similar trees consisting of two distinct main groups, and some intermediate isolates. BVV clustered within group "b" while NTV clustered together with the intermediate ophidian paramyxovirus isolate Crot2-OH90.
- Published
- 2001
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