1. Crocetin protects ultraviolet A-induced oxidative stress and cell death in skin in vitro and in vivo
- Author
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Kazuhiro Tsuruma, Naofumi Umigai, Mitsue Ishisaka, Takuya Ohba, Saori Tsujii, Takao Iwawaki, Koya Kubo, Masamitsu Shimazawa, and Hideaki Hara
- Subjects
Male ,0301 basic medicine ,Programmed cell death ,Ultraviolet Rays ,ved/biology.organism_classification_rank.species ,Crocetin ,Radiation-Protective Agents ,Pharmacology ,medicine.disease_cause ,Antioxidants ,Crocin ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Crocus sativus ,Autophagy ,medicine ,Animals ,Vitamin A ,Skin ,chemistry.chemical_classification ,Reactive oxygen species ,Cell Death ,biology ,ved/biology ,Crocus ,Endoplasmic Reticulum Stress ,biology.organism_classification ,Carotenoids ,Oxidative Stress ,030104 developmental biology ,chemistry ,Gardenia ,Biochemistry ,Apoptosis ,030220 oncology & carcinogenesis ,Lipid Peroxidation ,Reactive Oxygen Species ,Oxidative stress - Abstract
Crocetin, the aglycone of crocin, is a carotenoid found in fruits of gardenia (Gardeina jasminoides Ellis) and saffron (Crocus sativus L.). We investigated the protective effects of crocetin against ultraviolet-A (UV-A)-induced skin damage and explored the underlying mechanism. Human skin-derived fibroblasts cells (NB1-RGB) were damaged by exposure to UV-A irradiation (10J/cm(2)). Crocetin protected these cells against cell death and reduced the production of reactive oxygen species induced by UV-A irradiation. Crocetin treatment also suppressed induction of caspase-3 activation by UV-A irradiation. The effects of crocetin against oxidative stress were also examined by imaging of Keap1-dependent oxidative stress detector (OKD) mice. UV-A irradiation upregulated oxidative stress in the OKD mice skin, while crocetin administration (100mg/kg, p.o.) ameliorated this oxidative stress. Crocetin administration also decreased lipid peroxidation in the skin. These findings suggest that crocetin its observed protective effects against UV-A induced skin damage by reducing reactive oxygen species production and cell apoptosis.
- Published
- 2016