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The present experiment was carried out at Cotton Research Station, Vehari, Punjab during 2020-21. The objectives of this experiment were to assess the effect of cotton genotypes and sowing dates on yield and fibre quality parameters. Three upland cotton genotypes Viz., VH-402, VH-351 and VH-305 (check) were configured in a sub-plot using a randomized complete block design (RCBD) havinga split-plot arrangement with three replications. Eight sowing dates were arranged in the main plot starting from 1stMarch to 16th June with a uniform interval of two weeks. Data analysis depicted that sowing dates influenced all study traits significantly except for staple length. Similarly, genotypes and interactive effects were found significant. Genotype VH-351 yielded the highest (2523.3 kg ha-1) when sown in 1stMarch, while VH-402 gave the lowest seed cotton yield (209 kg ha-1) on the planting date of 16th June. The finest lint-bearing micronaire value (3.9656) was obtained by normal season sowing in 1stMay. Late sowing on 16th June resulted in coarse lint with (4.8189) micronaire value. Tough staple was found (33.511 g tex-1) at 1st March sowing, while frail staple (28.856 g tex-1) resulted in 16th May planting. In prevailing climatic conditions sowing of cotton on the earliest dates gave optimum seed cotton yield in South Punjab, while June sowing was proved uneconomical. [ABSTRACT FROM AUTHOR]