1. Troxerutin attenuates myocardial cell apoptosis following myocardial ischemia‐reperfusion injury through inhibition of miR‐146a‐5p expression.
- Author
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Shu, Liliang, Zhang, Wanzhe, Huang, Gongcheng, Huang, Chen, Zhu, Xiaohua, Su, Gang, and Xu, Jing
- Subjects
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REPERFUSION injury , *CORONARY disease , *HEART cells , *BIOFLAVONOIDS , *HYPOXEMIA , *POLYMERASE chain reaction , *HEMODYNAMICS , *CREATINE kinase - Abstract
The aim of the current study was to investigate the effects and the underlying mechanisms of troxerutin on myocardial cell apoptosis during ischemia‐reperfusion (I/R) injury. Hypoxia/reoxygenation (H/R) model in neonatal rat cardiomyocytes, and I/R model in rats, were established following troxerutin preconditioning. The quantitative real‐time polymerase chain reaction analysis was performed to examine the messenger RNA miR‐146a‐5p expression in cardiomyocytes and myocardial tissues. Hemodynamic parameters and serum creatine kinase, lactate dehydrogenase, tumor necrosis factor‐α, and interleukin‐10 were evaluated. Infarct size was examined by 2,3,5‐triphenyltetrazolium chloride staining. Besides, myocardial apoptosis was detected by terminal deoxynucleotidyl transferase (dUTP) nick end labeling (TUNEL) assay. Western blot analysis was performed to determine the protein levels of caspase‐3, Bax, and Bcl‐2. The results showed that, troxerutin decreased rat cardiomyocyte apoptosis during H/R injury. Furthermore, the antiapoptotic effect of troxerutin against I/R injury was mediated by miR‐146a‐5p downregulation. In vivo experiments suggested that troxerutin alleviated myocardial I/R injury in rats via inhibition of miR‐146a‐5p. In conclusion, troxerutin exerted cardioprotective effects during I/R injury by downregulating miR‐146a‐5p. In this study, we examined for the first time, the underlying mechanism through which troxerutin exert neuroprotective effect via regulating specific microRNAs. We concluded that, troxerutin alleviated myocardial ischemia‐reperfusion injury by downregulating miR‐146a‐5p. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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