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Aim: The OA-1000 (Tomey, Japan) is a new optical biometer, which measures axial length (AL), anterior chamber depth (ACD), and central corneal thickness (CT) utilizing optical interference technology. The aim of this study was to prove the reproducibility which is considered fundamental for other clinical investigations., Methods: 55 healthy volunteers were enrolled in this study. For each measurement of AL, ACD, and CT the biometer is grabbing a sequence of 10 shots and mean value (mean) and standard deviation (SD) are displayed. Five consecutive measurements were performed and average and standard deviation were assessed. Cronbach's α was derived as a quality measure for reproducibility., Results: For AL measurement Cronbach's α was 1.000, for CT 0.999, and for ACD 0.979, respectively. Mean value for AL was 23.36 ± 1.03 mm, for ACD it was 3.60 ± 0.687 mm, and for CT it was 552.08 ± 29.70 μm, respectively. Standard deviation for AL was 0.013 ± 0.022 mm, for ACD 0.09 ± 0.11 mm, and for CT 2.18 ± 1.75 μm. One correlation was found between mean values for AL and ACD (R = 0.388, P = 0.005); no other correlations were found between mean values or values of standard deviation of AL, ACD, or CT., Conclusion: The OA-1000 shows an excellent reproducibility for measurement of AL, ACD, and CT and can be used in clinical practice.