1. Boron-Salphen Conjugate based Molecular Probe Exhibiting Fluorescence On-Off-On Response in Detection of Cu 2+ and ATP through Displacement Approach.
- Author
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Bhandari R, Rai R, Kaleem M, Pratap R, Shraogi N, Patnaik S, Bhattacharya S, and Misra A
- Subjects
- Humans, HeLa Cells, Spectrometry, Fluorescence, Boron chemistry, Fluorescence, Molecular Structure, Copper chemistry, Adenosine Triphosphate analysis, Fluorescent Dyes chemistry, Fluorescent Dyes chemical synthesis
- Abstract
Synthesis and photophysical properties of a fluorescent probe HBD is described. Probe upon interaction with metal ions, anions and nucleoside pyrophosphates (NPPs) showed fluorescence quenching with Cu
2+ due to chelation enhanced quenching effect (CHEQ). Moreover, interaction of ensemble HBD.Cu2+ with anions and NPPs showed fluorescence "turn-On" response with ATP selectively. "On-Off-On" responses observed with Cu2+ and ATP is attributed to an interplay between ESIPT and TICT processes. Cyclic voltammogram of probe exhibited quasi-reversible redox behaviour with three oxidation and two reduction potentials and the change in band gaps of probe suggested the interaction with Cu2+ and ATP. The 2 : 1 and 1 : 1 binding stoichiometry for an interaction between probe and Cu2+ (LOD, 62 nM) and ensemble, HBD.Cu2+ with ATP (LOD, 0.4 μM) respectively are realised by Job's plot and HRMS data. Cell imaging studies carried out to detect Cu2+ and ATP in HeLa cells. Also, the output emission observed with Cu2+ and ATP is utilized to construct an implication (IMP) logic gate. Test paper strips showed naked-eye visible color responses to detect Cu2+ and ATP. In real water samples probe successfully detected copper (0.03 μM) between 5-6.5 ppb level (ICP-MS method)., (© 2024 Wiley-VCH GmbH.)- Published
- 2024
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