1. 缺氧预处理对神经干细胞间隙连接蛋白的表达及半通道功能的影响.
- Author
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马伟康, 刘庆鹏, 刘凯, 张雷, 王立春, 韩竹, 刘亮, 李琛, and 孙崇毅
- Subjects
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NEURAL stem cells , *GTPASE-activating protein , *LASER microscopy , *HYPOXEMIA , *CONTROL groups - Abstract
Objective: Determine the expression of gap junction protein and the changes of hemichannel function after hypoxia preconditioning of neural stem cells, which provided a theoretical basis for exploring the communication between neural stem cells after hypoxia preconditioning. Create a standardized preconditioning procedure and reproducibly expose the NSCs to hypoxia. Explore the best time for neural stemcells to be exposed to hypoxia without increasing necrosis. Methods: The expression of cx43, cx36, and cx47 in the neural stem cells of the control group and the hypoxia-preconditioned neurons were determined by immunohistochemical method at 1 h, 4 h, 8 h, and 12 h, respectively. The calcein dye efflux experiments was observed by laser confocal laser scanning microscopy to observe changes in hemichannell function. Results: Compared with the control group (1.03+0.14%), the necrosis rate of neural stem cells in 8 hours (3.05+0.40%) and 12 hours (5.51+0.31%) exposed to 0.5% oxygen was significantly higher (P<0.05). Compared with the control group, the expression of cx43, Cx36 and Cx47 in neural stem cells increased significantly after hypoxic preconditioning for 4, 8 and 12 hours (P<0.05). Hypoxia pretreatment group had significantly enhanced hemichannel filtration function compared with the control group and the hemichannel blocker group. Conclusions: Hypoxic preconditioning can improve the expression of connexin and the half-channel filtration function of neural stem cells in a time-dependent manner. The optimal time for neural stem cells to be exposed to hypoxia without causing significant increase in necrosis is 4 hours. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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