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Studies have reported the incorporation of microorganisms into cement to promote the formation of calcium carbonate in cracks of concrete, a process known as biomineralization. The paper aims to improve the process of the cascade system for biomineralization in cement by identifying the best hydrodynamic conditions in a reaction cell in order to increase the useful life of concrete structures and, therefore, bring energy and environmental benefits. Two central composite rotatable designs were used to establish the positioning of the air inlet and outlet in the lateral or upper region of the geometry of the reaction cell. The geometries of the reaction cell were constructed in SOLIDWORKS®, and computational fluid dynamics was performed using the Flow Simulation tool of the same software. The results were submitted to statistical analysis. The best combination of meshes for the simulation was global mesh 4 and local mesh 5. The statistical analysis applied to gas velocity and pressure revealed that air flow rate was the factor with the greatest sensitivity, with R2 values up to 99.9%. The geometry with the air outlet and inlet in the lateral region was considered to be the best option.

Anthropogenic and natural actions cause internal and external fractures in concrete. To recover these structures, bio-concretes have been developed with bacteria of the genus Bacillus. These microorganisms consume calcium lactate, synthesize calcium carbonate and biomineralize CaCO3 crystals within the structures of concrete. The aim of the present study was to construct equipment, denominated “Cascade System for Biomineralization in Cement” (CSBC), to determine the limiting velocity of the biomineralization of CaCO3. The construction of the equipment took into consideration chemical and biochemical phenomena responsible for biomineralization. Parts made with 3D printing and a circuit with Arduino UNO R3 board were used in the assembly of the system. The prototype proved to be stable and can be considered a promising tool for future application in research of the regeneration of reinforced concreted in a practical, fast and economical way, especially to the energy sector.

The production of cement accounts for 5 to 7% of carbon dioxide emissions in the world, and its broad-scale use contributes to climate imbalance. As a solution, biotechnology enables the cultivation of bacteria and fungi for the synthesis of calcium carbonate as one of the main constituents of cement. Through biomineralization, which is the initial driving force for the synthesis of compounds compatible with concrete, and crystallization, these compounds can be delivered to cracks in concrete. Microencapsulation is a method that serves as a clock to determine when crystallization is needed, which is assisted by control factors such as pH and aeration. The present review addresses possibilities of working with bioconcrete, describing the composition of Portland cement, analysis methods, deterioration, as well as environmental and energetic benefits of using such an alternative material. A discussion on carbon credits is also offered. The contents of this paper could strengthen the prospects for the use of self-healing concrete as a way to meet the high demand for concrete, contributing to the building of a sustainable society.