4 results on '"Riccio AM"'
Search Results
2. Preliminary evidence for 'aberrant' expression of the leukocyte integrin LFA-1 (CD11a/CD18) on conjunctival epithelial cells of patients with mite allergy.
- Author
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Pesce G, Ciprandi G, Buscaglia S, Fiorino N, Salmaso C, Riccio AM, Canonica GW, and Bagnasco M
- Subjects
- Adult, Animals, Antibodies, Monoclonal immunology, Chronic Disease, Conjunctivitis, Allergic etiology, Conjunctivitis, Allergic pathology, Epithelial Cells immunology, Epithelial Cells metabolism, Epithelial Cells pathology, Female, Fluorescent Antibody Technique, Indirect, Gene Expression, Humans, Immunoenzyme Techniques, Inflammation, Intercellular Adhesion Molecule-1 metabolism, Lymphocyte Function-Associated Antigen-1 genetics, Male, Mice, Middle Aged, Pollen, Rhinitis, Allergic, Perennial etiology, Rhinitis, Allergic, Seasonal immunology, Time Factors, Conjunctiva pathology, Conjunctivitis, Allergic immunology, Lymphocyte Function-Associated Antigen-1 biosynthesis, Mites immunology, Rhinitis, Allergic, Perennial immunology
- Abstract
Background: We have previously shown aberrant expression of the 'leukocyte' integrin LFA-1 on epithelial cells in chronic autoimmune thyroiditis. In the present study we investigated whether conjunctival epithelial cells, which bear the adhesion molecule ICAM-1 on their surface during allergic inflammation, may also aberrantly express its natural ligand, the 'leukocyte' integrin LFA-1., Methods: We studied 13 patients with rhinoconjunctivitis allergic to mites, chronically exposed to the allergen, 11 patients allergic to pollen tested out of the pollen season and 8 normal volunteers. Single and double immunocytochemical staining of conjunctival smears was employed., Results: LFA-1 staining on epithelial cells was demonstrated in 12/13 patients allergic to mites and not in normal controls or in patients allergic to pollen tested out of the pollen season. The epithelial localization of LFA-1 was confirmed by double staining with anti-LFA-1 and anti-cytokeratin antibodies (both immunocytochemical and immunofluorescence)., Conclusions: Coexpression of LFA-1 and ICAM-1 during persistent allergen stimulation may be relevant for interaction between epithelial cells and activated effector cells, such as eosinophils, which bear on their surface both ICAM-1 and its beta2 integrin ligands., (Copyright 2001 S. Karger AG, Basel)
- Published
- 2001
- Full Text
- View/download PDF
3. In situ hybridization analysis of ICAM-1 (CD54) mRNA on conjunctival epithelium during allergic inflammation.
- Author
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Bagnasco M, Pesce G, Fiorino N, Riccio AM, Ciprandi G, Buscaglia S, and Canonica GW
- Subjects
- 3T3 Cells, Adult, Animals, Cell Line, Epithelium immunology, Female, Humans, In Situ Hybridization, Male, Mice, Middle Aged, Nasal Cavity immunology, RNA, Messenger analysis, Conjunctiva immunology, Conjunctivitis, Allergic immunology, Intercellular Adhesion Molecule-1 biosynthesis
- Abstract
Background: The intercellular adhesion molecule ICAM-1 has been detected by immunohistochemical methods on epithelial cells of the conjunctiva and nose during allergic inflammation., Objective: The aim of the present study was to evaluate whether ICAM-1 expression on conjunctival epithelium derives from endogenous synthesis or is merely due to passive uptake of soluble ICAM-1 released from inflammatory cells., Methods: In situ hybridization was performed using a 3' end dygoxygenin-labelled specific DNA oligonucleotide probe on fixed conjunctival smears from allergic subjects challenged with, or naturally exposed to the allergen, and from healthy subjects. Immunocytochemistry for ICAM-1 was performed by alkaline phosphatase antialkaline phosphatase., Results: In allergic patients, both naturally exposed to the allergen and after specific challenge, a clear hybridization pattern on epithelial cells was apparent. Out of allergen exposure, some symptomfree pollinosic subjects, as well as a few healthy volunteers showed mild ICAM-1 mRNA cytoplasmic staining in the absence of immunohistochemically detectable ICAM-1. This finding may explain the very early appearance of ICAM-1 on conjunctival epithelium following specific challenge in allergic individuals., Conclusions: These results indicate that the presence of ICAM-1 on conjunctival epithelium during allergic inflammation derives from endogenous synthesis and not from uptake of soluble ICAM-1.
- Published
- 1997
4. Antiallergic activity of topical lodoxamide on in vivo and in vitro models.
- Author
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Ciprandi G, Buscaglia S, Catrullo A, Paolieri F, Riccio AM, Fiorino N, and Canonica GW
- Subjects
- Adolescent, Adult, Cell Line, Conjunctiva cytology, Conjunctiva drug effects, Conjunctiva metabolism, Cross-Over Studies, Double-Blind Method, Epithelium drug effects, Epithelium metabolism, Female, Humans, Intercellular Adhesion Molecule-1 biosynthesis, Male, Middle Aged, Oxamic Acid pharmacology, Oxamic Acid therapeutic use, Conjunctivitis, Allergic chemically induced, Conjunctivitis, Allergic drug therapy, Oxamic Acid analogs & derivatives
- Abstract
Lodoxamide is an antiallergic drug acting as a mast-cell stabilizer, which is effective in the treatment of allergic conjunctivitis. The study aimed to evaluate the effect of lodoxamide eye-drops on the inflammatory early-phase reaction (EPR) changes induced by allergen-specific conjunctival challenge (ASCC). This was a cross-over, double-blind, placebo-controlled, randomized study, including 10 outpatients suffering from allergic rhinoconjunctivitis due to Parietaria judaica. Patients received one drop of lodoxamide tromethamine 0.1% or placebo 30 min before each ASCC. Clinical evaluation and cytologic assessment were done at baseline and 30 min after each ASCC. Lodoxamide induced a reduction in total symptom score and hyperemia during the EPR (P < 0.05). Lacrimation, itching/burning, and eyelid swelling were only slightly (nonsignificantly) reduced. Lodoxamide induced a reduction in the total number of inflammatory cells and neutrophils during the EPR (P < 0.02). Eosinophil and lymphocyte number and ICAM-1 expression showed only a slight, not statistically significant decrease. Placebo did not affect the studied parameters. Lodoxamide reduced early clinical events and cellular changes after ASCC consistently with its activity as mast-cell stabilizer. Moreover, lodoxamide was able to downregulate in vitro ICAM-1 expression on the continuously cultured, differentiated conjunctival cell line WK. This was shown both in basal conditions (P < 0.05) and upon interferon-gamma stimulation (P < 0.03), although at high concentration.
- Published
- 1996
- Full Text
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