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Introduction Shift work can influence the health and well-being of individuals due to changes in biological rhythms, causing psychological and physical disruption, hampering family relationship, social and work ability. However, the shift work of auxiliary of direct / educational action of Private Social Solidarity Institution (PSSI) is essential to ensure continuity in the provision of the best hygienic and health care to the users of these institutions every day. Objectives The objective of this study was to evaluate the quality of life and the work capacity of Direct-Action Assistants (n = 29). Methodology Respondents were subjected a monitoring of the heart function PB – Blood Pressure for 24 hours and answered a questionnaire (Work Ability Index and Quality of Life Index). Results Of the 29 workers evaluated 90% were women and 10% men, aged 45.5 (± 10.47) years and exercise time of 7.1 (± 3.18) years. Physical examination revealed a mean 24 h Systolic Blood Pressure of 119.3 (± 11.8) mm Hg and 24 h Diastolic Blood Pressure of 72.6 (± 7.3) mm Hg. The mean Body Mass Index (BMI) (kg / m2) was 25.6 (± 3.5), and 24.1% of the participants were overweight and 13.7% obesity grade I. From this analysis, we found that overweight individuals have a tendency to increase the Blood Pressure compared to normal weight individuals. Although the working class has a good ability to work 69%, they are not optimistic about the future. Conclusion We concluded that workers can be adjusted the constant changes of schedules established by their institutions, and are able to carry out their work, however, individuals with 6 or more years of work in the present-day regime shifts already feel some symptoms of physical and psychological wear, and thus speeding up their aging. [ABSTRACT FROM AUTHOR]