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Here, to realize the simultaneous, simple, and highly sensitive detection for nitrofurazone and furazolidone, an immune-scaffold relying immunochromatographic assay was proposed. In this multi-detection immunochromatographic assay (MDICA), the immune-scaffold is composed of labeled anti-antibody IgG (Ab2) and two anti-analyte monoclonal antibodies (Abs1). A nanocomposite of MIL-101 (Cr)@AuNPs is used as the signal tag, where the unique optical property and biocompatibility of AuNPs and the protective effect of metal-organic framework can enable the nanocomposite possesses powerful signals, conjugating ability with antibodies and enhanced colloidal stability. In the immune-scaffold platform, the labeled Ab2 is the core trestle, on the one hand to exempt Ab1 from labeling requirement and offer strong detection signals, on the other hand to orient Abs1 to expose their antigen-binding sites as well as improve the identification and binding efficiency between antigen and antibody. Compared with randomly fixed antibody in the conventional probe, a 6-fold reduction of applied Ab1 amount can be achieved to produce the similar signal intensity in the immune-scaffold based method. In brief, the proposed MDICA system can effectively simplify the construction steps of MDICA, improve the Abs1 utilization efficiency, heighten analytical performances, and providing an efficient, valuable reference for the establishment of other MDICAs.

Folate conjugated trimethyl chitosan (FTMC)/graphene oxide (GO) nanocomplexes (FG NCs) prepared via electrostatic self-assembly were here developed as a targeted delivery vehicle for both doxorubicin (DOX) and plasmid DNA (pDNA). FG NCs exhibited the diameter of 112.0 nm and the thickness of 3.038 nm. Negligible cytotoxicity of FG NCs was observed in Hela and A549 cells. The higher uptake level in Hela cells with folate-receptor proved the targeting ability of FG NCs. Both DOX and pDNA could be loaded into FG NCs, wherein the loading capacity of DOX reached 30.9% and migration of pDNA could be completely retarded. Therefore, FG NCs could be served as a promising candidate for targeted delivery of both anticancer drugs and genes.