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Additional file 4: Figure S4. Graphical User Interface of Plength and its interference functionalities. Top panel: GUI with an uploaded image. Information on the file directory and image size are displayed. Bottom panel: Interference functionalities. (A) The four settings that can be changed, i.e. plant type, coleoptile or seedling, scale bar position and length, and minimum detection area. (B) Preprocessing. Before the analysis, the image can be cropped and the detected areas can be checked. (top) cropping tool; (bottom) color thresholding tool. (C) Postprocessing. The display after analysis completion. Detected regions are framed and numbered and the leaves are traced. For each detected area, the shoot, internode and leaf lengths are given.

Additional file 2: Figure S2. Extra information about the image processing pipeline in Plength. Top panel: Smoothing improving thresholding by homogenizing the colors, removal of the noise, and connection of edges. Middle panel: Segmentation, i.e. thresholding and partitioning of the image into individual plants. Bottom panel: Prior to feature extraction, skeletonization of the contour, conversion to a graph, and pruning. The red and green dots indicate nodes and connecting edges, respectively. Note that the initial graph consists of nine nodes, but the pruned graph of only of seven.