1. Computer modelling and visualization of active site of monoamine oxidases.
- Author
-
Veselovsky AV, Ivanov AS, and Medvedev AE
- Subjects
- Animals, Binding Sites physiology, Humans, Computer Simulation, Models, Chemical, Monoamine Oxidase chemistry, Monoamine Oxidase metabolism
- Abstract
In spite of significant progress in MAO research culminating in the crystallization of the MAO B, many structure-functional aspects of these enzymes still require better characterization. Computer modelling of the substrate/inhibitory binding region of the active site includes consensus overlay of several series of fully reversible and/or tightly bound inhibitors onto a rigid referent inhibitor(s). The shape of resultant mould obviously reflects spatial characteristic features of the substrate/inhibitor binding region. The comparison of the active site mould of MAO B with its crystal structure revealed correctness of this approach. The resultant moulds may be effectively used for virtual screening of molecular databases for new lead-structures. Using this approach we have found several selective MAO A inhibitors in databases and these compounds have never been tested for MAO inhibitory activity.
- Published
- 2004
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