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Background: Using artificial intelligence (AI) techniques for computational medical image analysis has shown promising results. However, colored images are often not readily available for AI analysis because of different coloring thresholds used across centers and physicians as well as the removal of clinical annotations. We aimed to develop an open-source tool that can adapt different color thresholds of HSV-colored medical images and remove annotations with a simple click. Materials and Methods: We built a function using MATLAB and used multi-center international shear wave elastography data (NCT 02638935) to test the function. We provide step-by-step instructions with accompanying code lines. Results: We demonstrate that the newly developed pre-processing function successfully removed letters and adapted different color thresholds of HSV-colored medical images. Conclusion: We developed an open-source tool for removing letters and adapting different color thresholds in HSV-colored medical images. We hope this contributes to advancing medical image processing for developing robust computational imaging algorithms using diverse multi-center big data. The open-source Matlab tool is available at https://github.com/cailiemed/image-threshold-adapting., Comment: An open-source tool that can adapt different color thresholds of HSV-colored medical images. The newly developed pre-processing Matlab function successfully works on multi-center, international shear wave elastography data (NCT 02638935). Step-by-step instructions with accompanying code lines were provided, easy to follow and reproduce