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2 results on '"Aouidat F"'

1. Galectin-1 protein modified gold (III)-PEGylated complex-nanoparticles: Proof of concept of alternative probe in colorimetric glucose detection.

Author

Liu Q, Aouidat F, Sacco P, Marsich E, Djaker N, and Spadavecchia J

Subjects
Glucose metabolism, Humans, Colorimetry methods, Galectin 1 chemistry, Galectin 1 metabolism, Glucose analysis, Gold chemistry, Metal Nanoparticles chemistry, Polyethylene Glycols chemistry
Abstract

Galectins (Gal) are a family of dimeric lectins, composed by two galactoside-binding sites implicated in the regulation of cancer progression and immune responses. In this study, we report for the first time the synthesis and the physical-chemical characterization of galectin-1-complex-gold COOH-terminated polyethlenglicole (PEG)-coated NPs (Gal-1 IN PEG-AuNPs) and their ability to recognize glucose in an aqueous solution with a concentration varying from 10 mM to 100 pM. The chemical protocol consistsof three steps: (i) complexation between galectin-1Gal-1 and tetrachloroauric acid (HAuCl 4 ) to form gold-protein grains; (ii) staking process of COOH-terminated polyethlenglicole molecules (PEG) onto Gal-1-Au complex and (iii) reduction of hybrid metal ions to obtain a colloidal stable solution. During the complexation, the spectral signatures related to the Gal-1 orientation on the gold surface have been found to change due to its protonation state. The effective glucose monitoring was detected by UV-vis, Raman spectroscopy and Transmission Electron Microscopy (TEM). Overall, we observed that the interaction is strongly dependent on the Gal-1 conformation at the surface of gold nanoparticles., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Published
2020
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2. Galectin-1 protein modified gold (III)-PEGylated complex-nanoparticles: Proof of concept of alternative probe in colorimetric glucose detection

Author

Jolanda Spadavecchia, Fatima Aouidat, Eleonora Marsich, Nadia Djaker, Pasquale Sacco, Qiqian Liu, Liu, Q., Aouidat, F., Sacco, P., Marsich, E., Djaker, N., Spadavecchia, J., Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques (CSPBAT), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Paris 13 (UP13)-Institut Galilée-Université Sorbonne Paris Cité (USPC)

Subjects
Galectin 1, Metal ions in aqueous solution, [SDV]Life Sciences [q-bio], Metal Nanoparticles, Nanoparticle, Protonation, 02 engineering and technology, AuNPs, 01 natural sciences, Polyethylene Glycols, Colloid and Surface Chemistry, 0103 physical sciences, PEG ratio, Galectin-1, Humans, Molecule, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Physical and Theoretical Chemistry, ComputingMilieux_MISCELLANEOUS, Galectin, Biomolecules colloid functionalization, Aqueous solution, LSPR, SERS, 010304 chemical physics, Chemistry, Surfaces and Interfaces, General Medicine, 021001 nanoscience & nanotechnology, Combinatorial chemistry, AuNP, 3. Good health, Glucose, Colloidal gold, Colorimetry, Gold, 0210 nano-technology, Biotechnology
Abstract

Galectins (Gal) are a family of dimeric lectins, composed by two galactoside-binding sites implicated in the regulation of cancer progression and immune responses. In this study, we report for the first time the synthesis and the physical-chemical characterization of galectin-1-complex-gold COOH-terminated polyethlenglicole (PEG)-coated NPs (Gal-1 IN PEG-AuNPs) and their ability to recognize glucose in an aqueous solution with a concentration varying from 10 mM to 100 pM. The chemical protocol consistsof three steps: (i) complexation between galectin-1Gal-1 and tetrachloroauric acid (HAuCl4) to form gold-protein grains; (ii) staking process of COOH-terminated polyethlenglicole molecules (PEG) onto Gal-1-Au complex and (iii) reduction of hybrid metal ions to obtain a colloidal stable solution. During the complexation, the spectral signatures related to the Gal-1 orientation on the gold surface have been found to change due to its protonation state. The effective glucose monitoring was detected by UV–vis, Raman spectroscopy and Transmission Electron Microscopy (TEM). Overall, we observed that the interaction is strongly dependent on the Gal-1 conformation at the surface of gold nanoparticles.

Published
2020
Full Text
View/download PDF

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