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Lateral flow assay (LFA) color signal quantification methods were developed by utilizing both International Commission on Illumination (CIE) LAB (CIELAB) color space and grayscale intensity differences. The CIELAB image processing procedure included calibration, test, control band detection, and color difference calculation, which can minimize the noise from the background. The LFA platform showcases its ability to accurately discern relevant colorimetric signals. The rising occurrence of infectious outbreaks from foodborne pathogens like Salmonella typhimurium presents significant economic, healthcare, and public health risks. The study introduces an aptamer-based lateral flow (ABLF) platform by using inkjet printing for specially detecting S. typhimurium. The ABLF utilized gold-decorated polystyrene microparticles, functionalized with specific S. typhimurium aptamers (Ps-AuNPs-ssDNA). The platform demonstrates a detection limit of 10 2 CFU mL -1 in buffer solutions and 10 3 CFU mL -1 in romaine lettuce tests. Furthermore, it sustained performance for over 8 weeks at room temperature. The ABLF platform and analysis methods are expected to effectively resolve the low-sensitivity problems of the former LFA systems and to bridge the gap between lab-scale platforms to market-ready solutions by offering a simple, cost-effective, and consistent approach to detecting foodborne pathogens in real samples., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)

The rising development of biosensors offers a great potential for health, food, and environmental monitoring. However, in many colorimetric platforms, there is a performance limitation stemming from the tendency of traditional Au nanoparticles toward nonspecific aggregation in response to changing ionic strength (salt concentration). This work puts forward a new type of colorimetric aptamer-functionalized labeling of microparticles, which allows to leverage an increase in ionic strength as a positive driver of enhanced detection performance of analytical targets. The resulting device is a cost-effective, instrument-free, portable, and reliable aptasensor that serves as basis for the fabrication of universal paper-based colorimetric platforms with the capability of multiplex, multireplicates and provides quantitative colorimetric detection. A controlled fabrication process was demonstrated by keeping 90% of the signal obtained from the as-fabricated devices ( n = 40) within ± 1 standard deviation (SD) (relative SD = 5.69%) and following a mesokurtic normal-like distribution ( p = 0.385). We propose for the first time a salt-induced aggregation mechanism for highly stable multilayered label particles (ssDNA-PEI-Au-PS) as the basis of the detection scheme. The use of DNA aptamers as capture biomolecules and PEI as an encapsulating agent allows for a sensitive and highly specific colorimetric response. As a proof of concept, multiplexed detection of mercury (Hg 2+ ) and arsenic (As 3+ ) was demonstrated. In addition, we introduced a robust image analysis algorithm for testing zone segmentation and color signal quantification that allowed for analytical detection, reaching a limit of detection of 1 ppm for both targeted analytes, with enough evidence ( p > 0.05) to prove the high specificity of the fabricated device versus a pool of possible interferent ions.

In electrophotographic printing, a periodic clustered-dot halftone pattern is preferred for a smooth and stable result. In addition, the screen frequency should be high enough to minimize the visibility of the halftone textures and to ensure good detail rendition. However, at these frequencies, the halftone cell may contain too few pixels to provide a sufficient number of distinct gray levels. This will result in contouring and posterization. The traditional solution is to grow the clusters asynchronously within a repeating block of clusters known as a supercell. The growth of each individual cluster is governed by a microscreen. The order in which the clusters grow within the supercell is determined by a macroscreen. Typically, the macroscreen is a recursive pattern due to Bayer. In highlights and shadows, this ordering results in visible artifacts. Replacing the Bayer screen by a stochastic macroscreen eliminates these artifacts, but results in new artifacts. In this paper, we propose a new composite screen architecture that employs multiple microscreens and multiple macroscreens in the highlights and shadows. These screens are jointly designed by using the direct binary search (DBS) algorithm.

In this paper, we present a methodology to design filters for an imaging system to improve the accuracy of the spectral measurements for families of reflective surfaces. We derive the necessary and sufficient conditions that the sensor space of the system must obey in order to measure the spectral reflectance of the surfaces accurately. Through simulations, we show how these conditions can be applied to design filters using a set of sample spectral data acquired from extracted teeth. For this set of data, we also compare our results to those of Wolski's method, a conventional filter design method which produces filters that recover tristimulus values of surfaces accurately under several illuminants. We show that our method produces filters that capture the spectral reflectance better given the same number of measurements. The errors in predicting the color of the sample data are much lower under every test illuminant when the filters designed with our method are used.

Characterization of halftone texture is important for quantitative assessment of halftone quality. In this paper, we develop a new framework based on directional local sequency analysis and a filter bank structure. We decompose a halftone image into subband images, from which we can easily reconstruct the original halftone. Based on these subband images, we define the directional sequency spectrum which is analogous to the two-dimensional Fourier spectrum, and formulate several texture measures. Two test image sets are used to justify these measures.