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Organo chemo sensors were used to detect fluoride ions in water samples and were synthesized by mixing two compounds in a 1:1 ratio with constant stirring for two hours using a stirrer with the addition of acetic acid and ethanol. The products were checked for a single spot using TLC paper. The experiment was taken further for the detection of fluoride ions by making a solution of chemo sensor and water sample at a 1:2 ratio and checked using a spectrophotometer and colorimeter. The values of absorbance were then compared with the standard solution containing fluoride ions. The formation of H - F bonding between the chemo sensors and fluoride ions in the water samples was confirmed. The results indicate the presence of fluoride ions in terms of ppm in the different water samples taken. [ABSTRACT FROM AUTHOR]