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Medicinal plants have widely been used as a natural source of remedies for treating several diseases, including colorectal cancer. Ocimum minimum L. is a very important dietary plant used in traditional and modern medicine, due to its health beneficial effect realized by cytotoxic, proapoptotic, antioxidant/prooxidant, antiviral and antimicrobial activity. The biological activity of O. minimum flowers has been evaluated in HCT-116 colorectal carcinoma cells through antiproliferative activity by MTT assay, pro-apoptotic activity by AO/EB and concentrations of redox status parameters (O2·- and lipid peroxidation) by colorimetric methods. The protein expression of iNOS was analyzed by immunocytochemistry, while the antimigratory effect was measured by xCELLigence system. The treatment with O. minimum shows the antiproliferative, proapoptotic, impact on redox status parameters and antimigratory effect on HCT-116 cells. Based on obtained results, the pharmacological effect of O. minimum is evident against HCT-116 colorectal carcinoma cells, suggesting that this plant may be good start material for future anticancer therapy investigation. [ABSTRACT FROM AUTHOR]

This study evaluated the potential of antitumor activity of snake venom from Vipera ammodytes and L-amino acid oxidase from Crotalus adamanteus on different colorectal cancer cell lines through determination of cytotoxic activity by MTT assay, pro-apoptotic activity by acridine orange/ethidium bromide staining, and concentrations of redox status parameters (superoxide, reduced glutathione, lipid peroxidation) by colorimetric methods. The expression of genes involved in the biotransformation process and metabolite efflux was determined by qPCR method, while protein expression of glutathione synthetase and P-glycoprotein were analysed by immunocytochemistry. The analysis of cell death shows that snake venom dominantly leads cells to necrosis. Induction of apoptosis by L-amino acid oxidase was in correlation with oxidative disbalance in cancer cells. Gene expression profile of membrane transporters and CYP genes were different in each cell line and in correlation with their sensitivity of treatment. Our results show that L-amino acid oxidase from snake venom is a potent cytotoxic substance with pronounced pro-apoptotic activity. The inhibition of P-glycoprotein suggests that L-amino acid oxidase is a good substance for furter research of antitumor effect, with unexpressed potential for occurrence of drug resistance in vitro. [Display omitted] • LAAO from Crotalus adamanteus venom is a proapoptotic agent. • The mechanism of their proapoptotic effect was mediated by changes in redox status. • LAAO change expression of genes involved in biotransformation processes. • Our in vitro results do not indicate the possibility of resistance occurrence. [ABSTRACT FROM AUTHOR]