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1. Caffeic acid phenethyl ester (CAPE) reverses fibrosis caused by chronic colon inflammation in murine model of colitis.

Author

Tambuwala MM, Kesharwani P, Shukla R, Thompson PD, and McCarron PA

Subjects
Animals, Disease Models, Animal, Female, Mice, Mice, Inbred C57BL, Phenylethyl Alcohol pharmacology, Caffeic Acids pharmacology, Colitis pathology, Colon drug effects, Fibrosis pathology, Inflammation pathology, Phenylethyl Alcohol analogs & derivatives
Abstract

Fibrosis is known to be the hallmarks of chronic inflammation of the bowel. Epithelial damage due to inflammation compromises the barrier function of the gastrointestinal tract. This barrier dysfunction leads to further spread of inflammation resulting in a chronic state of inflammation. This chronic inflammation leads to development of fibrosis, which has very limited therapeutic options and usually requires surgical removal of the affected tissue. Our previous work has shown that Caffeic acid phenethyl ester (CAPE) is a naturally occurring anti-inflammatory agent, found in propolis, has been found to be protective in experimental colitis via enhancement of epithelial barrier function. However, the impact of CAPE on resolution of fibrosis in the long-term is unknown. The aim of this follow up study was to investigate the effect of CAPE on colon fibrosis in a chronic model of Dextran sulphate sodium induced colitis in mice. Dextran sulphate sodium (DSS) 2.5% w/v was administered in drinking water to induce colitis in C57/BL6 mice for 5 days on the 6th day DSS was stopped and test group mice were treated with intraperitoneal administration of CAPE (30 mg kg -1 day -1 ) for a further 7 days. Disease activity index (DAI) score, colon length and tissue histology and level of tissue fibrosis was observed. CAPE-treated mice had significantly lower levels of DAI, tissue inflammation scores and fibrosis as compared with control group. Our results show that CAPE is effective in resolving colon fibrosis in chronic inflammation. Thus, we can conclude CAPE could be a potential therapeutic agent for further clinical investigations for treatment of fibrosis in inflammatory bowel diseases in humans., (Copyright © 2018 Elsevier GmbH. All rights reserved.)

Published
2018
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2. In vivo treatment with the herbal phenylethanoid acteoside ameliorates intestinal inflammation in dextran sulphate sodium-induced colitis

Author

D. H. Paper, Juergen Schoelmerich, Florian Obermeier, Nadja Dunger, K. Menzel, Werner Falk, Gerhard Rogler, Martin Hausmann, K. Balan, and Hans H Herfarth

Subjects
Necrosis, Colon, medicine.medical_treatment, Immunology, Enzyme-Linked Immunosorbent Assay, Inflammatory bowel disease, Antioxidants, Mice, Glucosides, Phenols, Weight Loss, medicine, Animals, Immunology and Allergy, Mesenteric lymph nodes, Intestinal Mucosa, Colitis, Acute colitis, Peroxidase, Respiratory Burst, Mice, Inbred BALB C, business.industry, Macrophages, Dextran Sulfate, Interleukin, medicine.disease, Respiratory burst, Cytokine, medicine.anatomical_structure, Acute Disease, Chronic Disease, Animal Studies, Cytokines, Female, Lymph Nodes, Inflammation Mediators, medicine.symptom, business
Abstract

Summary Recently we demonstrated that in inflammatory bowel disease (IBD) macrophage-oxidative burst activity is increased and NADPH oxidase mRNA is induced. The herbal phenylethanoid acteoside isolated from Plantago lanceolata L. was shown to exhibit anti-oxidative potential. Using the dextran sulphate sodium (DSS)-induced colitis model, in this study we have assessed whether systemic application of acteoside affects colitis. Colitis was induced by DSS in Balb/c mice. Treatment with acteoside (120, 600 µg/mouse/day) was performed intraperitoneally. The colon lengths were determined. Colonic tissue was scored histologically (max. score 8) by a blinded investigator. T cells isolated from mesenteric lymph nodes (MLN) were stimulated with anti-CD3 antibody in the presence of interleukin (IL)-2 (final concentration 10 U/ml). After incubation for 24 h, IL-1β, IL-6, IL-12 tumour necrosis factor (TNF)-α and interferon (IFN)-γ levels in supernatants were analysed by the beadlyte® cytokine detection system. Histological scoring of colonic tissue revealed that application of acteoside was followed by a significantly improved histological score. In acute colitis the histological score was 3·2 with acteoside versus 5·2 with phosphate-buffered saline (PBS) (P

Published
2007