1. Bovine Meniscus Middle Zone Tissue: Measurement of Collagen Fibril Behavior During Compression.
- Author
-
Sizeland KH, Wells HC, Kirby NM, Hawley A, Mudie ST, Ryan TM, and Haverkamp RG
- Subjects
- Animals, Biomechanical Phenomena, Cattle, Collagen metabolism, Collagen Type I chemistry, Collagen Type I metabolism, Elasticity, Male, Scattering, Small Angle, Stress, Mechanical, Synchrotrons, X-Ray Diffraction, Collagen chemistry, Menisci, Tibial chemistry, Menisci, Tibial physiology
- Abstract
Background: Type I collagen is the major component of the extracellular matrix of the knee's meniscus and plays a central role in that joint's biomechanical properties. Repair and reconstruction of tissue damage often requires a scaffold to assist the body to rebuild. The middle zone of bovine meniscus is a material that may be useful for the preparation of extracellular matrix scaffolds., Methods: Here, synchrotron-based small-angle X-ray scattering (SAXS) patterns of bovine meniscus were collected during unconfined compression. Collagen fibril orientation, D-spacing, compression distance and force were measured., Results: The collagen fibrils in middle zone meniscal fibrocartilage become more highly oriented perpendicular to the direction of compression. The D-spacing also increases, from 65.0 to 66.3 nm with compression up to 0.43 MPa, representing a 1.8% elongation of collagen fibrils perpendicular to the compression., Conclusion: The elasticity of the collagen fibrils under tension along their length when the meniscus is compressed, therefore, contributes to the overall elastic response of the meniscus only under loads that exceed those likely to be experienced physiologically., Competing Interests: Richard G Haverkamp reports grants from Australian Synchrotron, during the conduct of the study. The authors report no other possible conflicts of interest in this work., (© 2020 Sizeland et al.)
- Published
- 2020
- Full Text
- View/download PDF