1. Diarrhea and colitis in mice require the Salmonella pathogenicity island 2-encoded secretion function but not SifA or Spv effectors.
- Author
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Fierer J, Okamoto S, Banerjee A, and Guiney DG
- Subjects
- ADP Ribose Transferases genetics, ADP Ribose Transferases metabolism, Alleles, Animals, Bacterial Proteins genetics, Cation Transport Proteins genetics, Cation Transport Proteins metabolism, Colon pathology, Feces chemistry, Female, Genetic Predisposition to Disease, Glycoproteins genetics, Glycoproteins metabolism, Macrophages physiology, Membrane Proteins genetics, Mice, Mice, Inbred BALB C, Salmonella genetics, Salmonella metabolism, Salmonella pathogenicity, Salmonella typhimurium metabolism, Salmonella typhimurium pathogenicity, Specific Pathogen-Free Organisms, Virulence genetics, Virulence Factors genetics, Virulence Factors metabolism, Bacterial Proteins metabolism, Colitis microbiology, Diarrhea microbiology, Membrane Proteins metabolism, Salmonella Infections, Animal microbiology, Salmonella typhimurium genetics
- Abstract
We investigated the roles of Salmonella pathogenicity island 2 (SPI-2) and two SPI-2 effectors in Salmonella colitis and diarrhea in genetically resistant BALB/c.D2(Slc11a1) congenic mice with the wild-type Nramp1 locus. Wild-type Salmonella enterica serovar Typhimurium 14028s caused a pan-colitis, and the infected mice developed frank diarrhea with a doubling of the fecal water content. An ssaV mutant caused only a 26% increase in fecal water content, without producing the pathological changes of colitis, and it did not cause weight loss over a 1-week period of observation. However, two SPI-2 effector mutants, the spvB and sifA mutants, and a double spvB sifA mutant caused diarrhea and colitis, even though the sifA mutant was sensitive to killing by bone marrow-derived macrophages from BALB/c.D2 mice and was severely impaired in extraintestinal growth but not in growth in the cecum. These results demonstrate that systemic S. enterica infection and diarrhea/colitis are distinct pathogenic processes and that only the former requires spvB and sifA.
- Published
- 2012
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