1. PMC-12, a Prescription of Traditional Korean Medicine, Improves Amyloid β-Induced Cognitive Deficits through Modulation of Neuroinflammation.
- Author
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Park, Min Young, Jung, Yeon Suk, Park, Jung Hwa, Choi, Young Whan, Lee, Jaewon, Kim, Cheol Min, Baek, Jin Ung, Choi, Byung Tae, and Shin, Hwa Kyoung
- Subjects
PHYTOTHERAPY ,COGNITION disorders diagnosis ,AMYLOIDOSIS diagnosis ,ALZHEIMER'S disease prevention ,PROTEINS ,NONSTEROIDAL anti-inflammatory agents ,QUANTITATIVE research ,CYCLOOXYGENASE 2 ,LEARNING ,PLANT extracts ,MICE ,CELL culture ,PHYSICIAN practice patterns ,ANIMAL experimentation ,ALTERNATIVE medicine ,DRUG prescribing ,INFLAMMATION - Abstract
PMC-12 is a prescription used in traditional Korean medicine that consists of a mixture of four herbal medicines, Polygonum multiflorum, Rehmannia glutinosa, Polygala tenuifolia, and Acorus gramineus, which have been reported to have various pharmacological effects on age-related neurological diseases. In the present study, we investigated whether PMC-12 improves cognitive deficits associated with decreased neuroinflammation in an amyloid-β-(Aβ-) induced mouse model and exerts the antineuroinflammatory effects in lipopolysaccharide-(LPS-) stimulated murine BV2 microglia. Intracerebroventricular injection of Aβ
25 -35 in mice resulted in impairment in learning and spatial memory, whereas this was reversed by oral administration of PMC-12 (100 and 500 mg/kg/day) in dose-dependent manners. Moreover, PMC-12 reduced the increase of Aβ expression and activation of microglia and astrocytes in the Aβ25 -35 -injected brain. Furthermore, quantitative PCR data showed that inflammatory mediators were significantly decreased by administration of PMC-12 in Aβ-injected brains. Consistent with the in vivo data, PMC-12 significantly reduced the inflammatory mediators in LPS-stimulated BV2 cells without cell toxicity. Moreover, PMC-12 exhibited anti-inflammatory properties via downregulation of ERK, JNK, and p38 MAPK pathways. These findings suggest that the protective effects of PMC-12 may be mediated by its antineuroinflammatory activities, resulting in the attenuation of memory impairment; accordingly, PMC-12 may be useful in the prevention and treatment of AD. [ABSTRACT FROM AUTHOR]- Published
- 2015
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